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Titolo:
GROWTH HORMONE-RECEPTOR MESSENGER-RNA IN THE RAT OVARY - REGULATION AND LOCALIZATION
Autore:
CARLSSON B; NILSSON A; ISAKSSON OGP; BILLIG H;
Indirizzi:
GOTHENBURG UNIV,DEPT PHYSIOL,POB 33031 S-40033 GOTHENBURG SWEDEN
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 1-2, volume: 95, anno: 1993,
pagine: 59 - 66
SICI:
0303-7207(1993)95:1-2<59:GHMITR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORCINE GRANULOSA-CELLS; SERUM BINDING-PROTEIN; INSULIN-LIKE; FACTOR-I; GENE-EXPRESSION; INDUCED DIFFERENTIATION; RIBONUCLEIC-ACID; SOMATOMEDIN-C; FEMALE RATS; HUMAN LIVER;
Keywords:
GROWTH HORMONE RECEPTOR MESSENGER RNA; BINDING PROTEIN; OVARY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
70
Recensione:
Indirizzi per estratti:
Citazione:
B. Carlsson et al., "GROWTH HORMONE-RECEPTOR MESSENGER-RNA IN THE RAT OVARY - REGULATION AND LOCALIZATION", Molecular and cellular endocrinology, 95(1-2), 1993, pp. 59-66

Abstract

The results of several reports indicate that GH can modulate ovarian function. In the present study, the expression of the growth hormone-receptor (GH-R) mRNA was studied in the rat ovary using an RNA probe corresponding to a part of the extracellular domain of the GH-R. The probe hybridized to two major transcripts with estimated sizes of 4.0 kb and 1.2 kb in RNA extracted from liver and ovary. Recently, these transcripts have been shown to encode the GH-R and the GH-binding protein (GH-BP). The ontogeny of the GH-R/GH-BP mRNA expression was studied using Northern blot analysis and a solution hybridization RNase protection assay. In the liver GH-R/GH-BP mRNA levels increased with age, while in the ovary, the levels decreased between 1 and 5 weeks of age. Hypophysectomy caused a decrease in GH-R/GH-BP gene expression in the ovary, an effect which could be partly reversed with a single injection of GH (2 mg/kg). No significant changes in the ovarian concentration ofGH-R/GH-BP transcripts were seen during the estrus cycle. Using in situ hybridization GH-R/GH-BP transcripts were found to be most abundantin follicles. Northern blot analysis of RNA extracted from isolated granulosa cells and corpora lutea showed that both these compartments contained GH-R and GH-BP mRNA, although more abundant in granulosa cells. Immunoreactive GH-R was detected in granulosa cells of healthy follicles, corpus luteum, and in the germinal epithelium. The results of the present study demonstrate that transcripts coding for the GH-R and the GH-BP are expressed in the follicles and, to a lesser extent, in the corpus luteum of the rat ovary. The levels of GH-R mRNA were found to be influenced by age and by GH treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:33:06