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Titolo:
MOLECULAR ANALYSIS OF 3 MAIZE 22 KDA AUXIN-BINDING PROTEIN GENES - TRANSIENT PROMOTER EXPRESSION AND REGULATORY REGIONS
Autore:
SCHWOB E; CHOI SY; SIMMONS C; MIGLIACCIO F; ILAG L; HESSE T; PALME K; SOLL D;
Indirizzi:
YALE UNIV,DEPT MOLEC BIOPHYS & BIOCHEM NEW HAVEN CT 06511 YALE UNIV,DEPT MOLEC BIOPHYS & BIOCHEM NEW HAVEN CT 06511 MAX PLANCK INST ZUCHTUNGSFORSCH W-5000 COLOGNE 30 GERMANY
Titolo Testata:
Plant journal
fascicolo: 3, volume: 4, anno: 1993,
pagine: 423 - 432
SICI:
0960-7412(1993)4:3<423:MAO3M2>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZEA-MAYS-L; PLASMA-MEMBRANE; ENDOPLASMIC-RETICULUM; CORN COLEOPTILES; PLANT GENES; RECEPTOR; IDENTIFICATION; SEQUENCE; ELEMENT; SIGNAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
E. Schwob et al., "MOLECULAR ANALYSIS OF 3 MAIZE 22 KDA AUXIN-BINDING PROTEIN GENES - TRANSIENT PROMOTER EXPRESSION AND REGULATORY REGIONS", Plant journal, 4(3), 1993, pp. 423-432

Abstract

The site 122 kDa auxin-binding proteins from maize are encoded by a small gene family comprising at least five members. Here the cloning and molecular analysis of the Zm-ERabp1, Zm-ERabp4, and Zm-ERabp5 genes is presented. All three encode 22-23 kDa proteins displaying a transitpeptide, a C-terminal KDEL sequence, as well as glycosylation and auxin-binding sites. The Zm-ERabp4 and Zm-ERabp5 genes are very similar. The Zm-ERabp1 gene encodes a related protein, but its promoter, leaderand signal peptide are very different. Northern analysis using gene-specific oligonucleotide probes indicates that Zm-ERabp4 is expressed in leaves and coleoptiles but weakly in roots, whereas Zm-ERabp5 expression is barely detectable in these tissues. RNA-PCR indicated that allthree genes are none the less expressed in many tissues. Primer-extension analysis revealed an unusually long (320 bases) Zm-ERabp1 leader containing an 80 codon ORF which, if expressed, would encode a positively charged protein with some similarity to transcription factors. In a transient promoter-reporter gene expression system using maize leaf protoplasts the Zm-ERabp1 promoter is more active than the Zm-ERabp4 and Zm-ERabp5 promoters. Promoter deletion analysis of Zm-ERabp1 has identified a negative regulatory sequence in a region from -364 bp and -130 bp, deletion of which results in about twofold higher expression. This region contains both enhancer- and G-box-related sequences. Deletion of -129 bp to +64 bp, which contains the TATA box and transcription start, results in a large decrease in expression.

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Documento generato il 27/11/20 alle ore 09:34:43