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Titolo:
SWELLING IN THE ISOLATED-PERFUSED CORNEA INDUCED BY 12(R)HYDROXYEICOSATETRAENOIC ACID
Autore:
EDELHAUSER HF; GEROSKI DH; WOODS WD; HOLLEY GP; LANIADOSCHWARTZMAN M;
Indirizzi:
EMORY UNIV,CTR EYE,DEPT OPHTHALMOL,1327 CLIFTON RD NE ATLANTA GA 30322 EMORY UNIV,SCH MED,DEPT OPHTHALMOL ATLANTA GA 30322 NEW YORK MED COLL,DEPT PHARMACOL VALHALLA NY 10595
Titolo Testata:
Investigative ophthalmology & visual science
fascicolo: 10, volume: 34, anno: 1993,
pagine: 2953 - 2961
SICI:
0146-0404(1993)34:10<2953:SITICI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARACHIDONIC-ACID; 12(R)-HYDROXYEICOSATRIENOIC ACID; METABOLITE; RABBIT; CYTOCHROME-P450; EPITHELIUM; OUABAIN;
Keywords:
CORNEAL ENDOTHELIUM; 12(R)HETE; CORNEAL SWELLING; NA/K ATPASE; ENDOTHELIAL PUMP; OUABAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
H.F. Edelhauser et al., "SWELLING IN THE ISOLATED-PERFUSED CORNEA INDUCED BY 12(R)HYDROXYEICOSATETRAENOIC ACID", Investigative ophthalmology & visual science, 34(10), 1993, pp. 2953-2961

Abstract

Purpose. To evaluate the effect of 12(R)hydroxyeicosatetraenoic acid (12(R)HETE) on corneal swelling when directly perfused to human and rabbit corneal endothelium. Methods. Excised rabbit and human corneas were mounted in the in vitro specular microscope and the endothelium wasperfused with 12(R)HETE at 10(-5), 10(-6), and 10(-7) mol/l. Both 12(R)HETE and 12(S)HETE were compared at equal molar (10(-6) mol/l) concentrations. The reversal of 12(R)HETE and ouabain corneal swelling was also compared. Endothelial permeability to carboxyfluorescein was measured after 12(R)HETE perfusion. High-performance liquid chromatographic analysis confirmed that 12(R)HETE remained in the perfusion media. Results. 12(R)HETE caused a dose-dependent corneal swelling of 25 +/- 2, 24 +/- 1, and 14 +/- 0.5 mum/hr at 10(-5), 10(-6), and 10(-7) mol/l,respectively. Equal molar concentrations (10(-6) mol/l) of 12(S)HETE did not cause corneal swelling. Removal of the 12(R)HETE from the perfusion media resulted in reversal of corneal swelling whereas corneal swelling induced by ouabain did not reverse after ouabain removal. 12(R)HETE (10(-6) mol/l) perfused to the human corneal endothelium inhibited temperature reversal corneal thinning when compared to the paired corneal endothelium perfused with BSS Plus (Alcon Laboratories, Inc., Fort Worth, TX). Na/K adenosine triphosphatase activity was inhibited by 10(-6) mol/l ouabain by 35%, 10(-6) mol/l 12(R)HETE by 54%, and 10(-6) mol/l 12(S)HETE by 0.5%. Endothelial permeability to carboxyfluorescein was unaffected by 12(R)HETE. Conclusion. 12(R)HETE causes cornealswelling by inhibiting endothelial pump function. This inhibition of transport appears to be at least partly mediated by inhibition of endothelial Na/K adenosine triphosphatase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 17:23:00