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Titolo:
CALCIUM-DEPENDENT CHLORIDE SECRETION ACROSS CULTURES OF HUMAN TRACHEAL SURFACE EPITHELIUM AND GLANDS
Autore:
YAMAYA M; OHRUI T; FINKBEINER WE; WIDDICOMBE JH;
Indirizzi:
UNIV CALIF SAN FRANCISCO,CARDIOVASC RES INST,DEPT PHYSIOL SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,CARDIOVASC RES INST,DEPT PHYSIOL SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,CYST FIBROSIS RES CTR SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT PATHOL SAN FRANCISCO CA 94143
Titolo Testata:
The American journal of physiology
fascicolo: 2, volume: 265, anno: 1993,
parte:, 1
pagine: 120000170 - 120000177
SICI:
0002-9513(1993)265:2<120000170:CCSACO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA-CL COTRANSPORT; CYSTIC-FIBROSIS; ION-TRANSPORT; INTRACELLULAR CALCIUM; POTASSIUM CHANNELS; AIRWAY EPITHELIUM; SODIUM-TRANSPORT; APICAL MEMBRANE; CELLS; BRADYKININ;
Keywords:
CYSTIC FIBROSIS; INTRACELLULAR FREE CALCIUM CONCENTRATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
M. Yamaya et al., "CALCIUM-DEPENDENT CHLORIDE SECRETION ACROSS CULTURES OF HUMAN TRACHEAL SURFACE EPITHELIUM AND GLANDS", The American journal of physiology, 265(2), 1993, pp. 120000170-120000177

Abstract

Surface epithelium and gland cells from human trachea were cultured on porous-bottom inserts and loaded with fura 2 to permit measurement of the intracellular calcium concentration ([Ca2+]i). Short-circuit current (I(SC)), an index of transepithelial active ion transport, was measured on cells from the same cultures. Surface epithelial [Ca2+]i of 82 +/- 15 nM was increased transiently by isoproterenol, histamine, and bradykinin with maximal increases of 88 +/- 17, 480 +/- 149, and 978+/- 214 nM (n = 15), respectively. Baseline [Ca2+]i in cultured glandcells of 68 +/- 11 nM was increased transiently by isoproterenol, histamine, methacholine, and bradykinin with maximal increases of 105 +/-19, 233 +/- 47, 327 +/- 121, and 634 +/- 151 nM (n = 17-21), respectively. In both cell types, mediators that increased [Ca2+]i also increased I(SC) with a time course identical to the increase in [Ca2+]i. Pretreatment with the calcium chelator, 1,2-bis-(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid, acetoxymethyl ester (BAPTA-AM), had no effect on basal I(SC) or transepithelial resistance but markedly inhibited both the I(SC) and [Ca2+] i responses to agonists. Forskolin (10(-5) M), 3-isobutyl-1-methylxanthine (10(-3) M), dibutyryl adenosine 3',5'-cyclic monophosphate (10(-3) M), and 8-(4-chlorophenylthio)-cAMP (10(-3) M) had no or only trivial effects on I(SC) and [Ca2+]i. We suggest that mediators increase I(SC) across human airway epithelium by activating Ca-dependent basolateral K channels, resulting in hyperpolarization and an increased driving force for Cl exit through apical membrane Cl channels.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:26:28