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Titolo:
COOPERATIVE HOMODIMERIC HEMOGLOBIN FROM SCAPHARCA-INAEQUIVALVIS - CDNA CLONING AND EXPRESSION OF THE FULLY FUNCTIONAL PROTEIN IN ESCHERICHIA-COLI
Autore:
GAMBACURTA A; PIRO MC; ASCOLI F;
Indirizzi:
TOR VERGATA UNIV,DEPT EXPTL MED & BIOCHEM SCI,VIA O RAIMONDO I-00173 ROME ITALY TOR VERGATA UNIV,DEPT EXPTL MED & BIOCHEM SCI,VIA O RAIMONDO I-00173 ROME ITALY
Titolo Testata:
FEBS letters
fascicolo: 1, volume: 330, anno: 1993,
pagine: 90 - 94
SICI:
0014-5793(1993)330:1<90:CHHFS->2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLLUSK ANADARA-TRAPEZIA; AMINO-ACID-SEQUENCE; ESCHERICHIA-COLI; BIVALVE MOLLUSK; DIMERIC HEMOGLOBIN; TETRAMERIC HEMOGLOBINS; OXYGEN EQUILIBRIA; LIGAND-BINDING; MYOGLOBIN; CHAIN;
Keywords:
GLOBIN CDNA; REVERSE TRANSCRIPTASE-PCR; EXPRESSION IN ESCHERICHIA-COLI; RECOMBINANT HEMOGLOBIN; SCAPHARCA-INAEQUIVALVIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
A. Gambacurta et al., "COOPERATIVE HOMODIMERIC HEMOGLOBIN FROM SCAPHARCA-INAEQUIVALVIS - CDNA CLONING AND EXPRESSION OF THE FULLY FUNCTIONAL PROTEIN IN ESCHERICHIA-COLI", FEBS letters, 330(1), 1993, pp. 90-94

Abstract

The overexpression of the fully functional, cooperative homodimeric hemoglobin of the bivalve mollusc, Scapharca inaequivalvis, has been accomplished in E. coli from its cDNA. The latter was isolated by PCR amplification of total RNA and sequenced. The cDNA-derived sequence differed by a single amino acid when compared to that previously obtained from purified protein. Interest in this hemoglobin resides in the unique assemblage of the two identical subunits, with the heme groups facing each other in the inside of the molecule, opposite to that occurring in vertebrate hemoglobins. The results presented here are the basis for future studies of structure/function relationships by site directed mutagenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:10:02