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Titolo:
EXPRESSION OF E-CADHERIN OR P-CADHERIN IS NOT SUFFICIENT TO MODIFY THE MORPHOLOGY AND THE TUMORIGENIC BEHAVIOR OF MURINE SPINDLE CARCINOMA-CELLS - POSSIBLE INVOLVEMENT OF PLAKOGLOBIN
Autore:
NAVARRO P; LOZANO E; CANO A;
Indirizzi:
UAM,FAC MED,CSIC,INST INVEST BIOMED,DEPT BIOQUIM,ARTURO DUPERIER 4 E-28029 MADRID SPAIN UAM,FAC MED,CSIC,INST INVEST BIOMED,DEPT BIOQUIM,ARTURO DUPERIER 4 E-28029 MADRID SPAIN
Titolo Testata:
Journal of Cell Science
, volume: 105, anno: 1993,
parte:, 4
pagine: 923 - 934
SICI:
0021-9533(1993)105:<923:EOEOPI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE EPIDERMAL-KERATINOCYTES; UVOMORULIN-CATENIN COMPLEX; TUMOR SUPPRESSOR GENES; MOLECULE E-CADHERIN; ADHESION MOLECULE; CYTOPLASMIC DOMAIN; ALPHA-CATENIN; BETA-CATENIN; N-CADHERIN; RAS GENE;
Keywords:
CADHERINS; PLAKOGLOBIN; EPIDERMAL CARCINOGENESIS; MORPHOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
P. Navarro et al., "EXPRESSION OF E-CADHERIN OR P-CADHERIN IS NOT SUFFICIENT TO MODIFY THE MORPHOLOGY AND THE TUMORIGENIC BEHAVIOR OF MURINE SPINDLE CARCINOMA-CELLS - POSSIBLE INVOLVEMENT OF PLAKOGLOBIN", Journal of Cell Science, 105, 1993, pp. 923-934

Abstract

Transfection of E- and P-cadherin CDNA has been carried out in murinespindle carcinoma cells previously shown to be deficient in both cadherins (Navarro et al., J. Cell Biol. 115, 517-533, 1991). High levels of expression of E- or P-cadherin do not significantly affect the fibroblastic morphology of the parental spindle cells. In addition, the tumorigenic behavior of these highly malignant cells is not influenced by the ectopic expression of either cadherin. Nevertheless, a fraction of the exogenous cadherins is able to associate to detergent-insolublecomponents of the transfectant cells, and the expression of the exogenous E-cadherin confers Ca2+-dependent aggregation on the spindle transfectants in an in vitro assay. Immunoprecipitation analysis of the cadherin-catenin complex of the transfectants revealed that the ectopic E-cadherin associates with the alpha- and beta-catenin proteins. However, the gamma-catenin/plakoglobin component could not be detected in the E-cadherin immunocomplexes of the spindle transfectant cells, in contrast to the epithelial cells where the three catenins appeared to beassociated with E-cadherin. The lack of association of gamma-catenin is correlated with very low levels of plakoglobin in whole cell extracts of the parental spindle cells. These results indicate that the association of E-cadherin with the alpha- and beta-catenin components is not sufficient to promote a fibroblastoid-epithelial conversion of highly malignant spindle cells. The presence of plakoglobin could be required for the proper organization of E-cadherin in the transfectant cells in order to acquire an epithelioid phenotype.

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Documento generato il 05/07/20 alle ore 06:05:34