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Titolo:
OLIGODEOXYNUCLEOTIDES INTERACT WITH RECOMBINANT CD4 AT MULTIPLE SITES
Autore:
YAKUBOV L; KHALED Z; ZHANG LM; TRUNEH A; VLASSOV V; STEIN CA;
Indirizzi:
COLUMBIA UNIV COLL PHYS & SURG,DEPT MED NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG,DEPT MED NEW YORK NY 10032 SMITHKLINE BEECHAM PHARMACEUT,MOLEC GENET KING OF PRUSSIA PA 19406 NOVOSIBIRSK BIOORGAN CHEM INST NOVOSIBIRSK 630090 RUSSIA
Titolo Testata:
The Journal of biological chemistry
fascicolo: 25, volume: 268, anno: 1993,
pagine: 18818 - 18823
SICI:
0021-9258(1993)268:25<18818:OIWRCA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; ANTI-HIV AGENT; BINDING-SITE; DEXTRAN SULFATE; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES; SYNCYTIUM FORMATION; AURINTRICARBOXYLIC ACID; PENTOSAN POLYSULFATE; HIV-GP120 BINDING; ATOMIC-STRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
L. Yakubov et al., "OLIGODEOXYNUCLEOTIDES INTERACT WITH RECOMBINANT CD4 AT MULTIPLE SITES", The Journal of biological chemistry, 268(25), 1993, pp. 18818-18823

Abstract

Phosphodiester oligodeoxynucleotides bearing the 5'-alkylating moiety4-(N-2-chloroethyl-N-methyl)aminobenzylamine specifically modify recombinant soluble CD4 (rsCD4) in solution. This reaction is saturable with respect to the alkylating oligonucleotide reagent. The existence ofat least two binding sites, with different affinities, on the rsCD4 molecule, were demonstrated. The values of apparent K(d) for the sites are approximately 0.1 and 1 muM. The existence of two sites was confirmed by electrophoretic analysis of the modified protein, in which two distinct gel bands were seen. The modification is inhibited hy excess non-alkylating oligonucleotide, as well as by phosphorothioate oligonucleotides. Quantitative estimates of the competition constants (K(c)),for the binding of these competitors of the binding of the alkylatingoligonucleotide reagent with rsCD4, have been made. By use of this method, several anionic dyes as well as potential anti-HIV therapeutic agents were also demonstrated to interact with rsCD4. Phosphorothioate oligonucleotides also inhibit binding of rsCD4 with the monoclonal antibody L71.1.1 This monoclonal antibody recognizes the CDR3-like loop (D1 domain) of the rsCD4 molecule. Thus, oligonucleotide binding sites exist on two remote regions (i.e. both the CDR2- and CDR3-like loops) of the D1 domain of CD4.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:50:29