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Titolo:
COORDINATED DECREASES IN RIBOSOMAL-RNA GENE-TRANSCRIPTION FACTORS ANDRIBOSOMAL-RNA SYNTHESIS DURING MUSCLE-CELL DIFFERENTIATION
Autore:
LARSON DE; XIE WQ; GLIBETIC M; OMAHONY D; SELLS BH; ROTHBLUM LI;
Indirizzi:
UNIV GUELPH,DEPT MOLEC BIOL & GENET GUELPH N1G 2W1 ONTARIO CANADA GEISINGER MED CLIN,WEIS CTR RES DANVILLE PA 17822
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 17, volume: 90, anno: 1993,
pagine: 7933 - 7936
SICI:
0027-8424(1993)90:17<7933:CDIRGF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA POLYMERASE-I; RAT RIBOSOMAL DNA; L6E9 MYOBLAST DIFFERENTIATION; MESSENGER-RNA; FACTOR UBF; BINDING-PROTEIN; MOUSE RDNA; PROMOTER; IDENTIFICATION; INITIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
D.E. Larson et al., "COORDINATED DECREASES IN RIBOSOMAL-RNA GENE-TRANSCRIPTION FACTORS ANDRIBOSOMAL-RNA SYNTHESIS DURING MUSCLE-CELL DIFFERENTIATION", Proceedings of the National Academy of Sciences of the United Statesof America, 90(17), 1993, pp. 7933-7936

Abstract

rRNA synthesis decreases significantly during the differentiation of rat L6 myoblasts to myotubes. Nuclear run-on assays demonstrated that the decrease was attributable to decreased rates of rRNA gene transcription. Immunoblot analysis indicated a marked reduction in amounts of the RNA polymerase I transcription factors UBF1 and UBF2 (upstream binding factors 1 and 2, respectively). The levels of these factors dropped in parallel with the down-shift in rRNA gene transcription. The amount of UBF does not fall due to a general decrease in cellular protein, as myosin heavy-chain protein accumulates markedly during this same time. RNA blots of total RNA isolated from myoblasts and differentiating myotubes showed a decrease in the mRNA for UBF, at the same time the mRNA for myogenin was accumulating. The downshift in UBF mRNA levelspreceded the decrease in the protein levels for UBF. There have been reports that the acute response of the rRNA gene transcription system to physiological signals in many systems involves an RNA polymerase I-associated factor. However, our results imply that the regulation of rRNA gene DNA transcription in response to physiological processes, such as differentiation, may involve multiple regulatory path ways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 12:29:22