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Titolo:
IDENTIFICATION OF RENAL SYMPATHETIC PREGANGLIONIC NEURONS IN HAMSTERSUSING TRANSSYNAPTIC TRANSPORT OF HERPES-SIMPLEX TYPE-1 VIRUS
Autore:
DEHAL NS; DEKABAN GA; KRASSIOUKOV AV; PICARD FJ; WEAVER LC;
Indirizzi:
JOHN P ROBARTS RES INST,100 PERTH DR,POB 5015 LONDON N6A 5K8 ON CANADA JOHN P ROBARTS RES INST,100 PERTH DR,POB 5015 LONDON N6A 5K8 ON CANADA UNIV WESTERN ONTARIO,DEPT PHYSIOL LONDON N6A 3K7 ONTARIO CANADA UNIV WESTERN ONTARIO,DEPT MICROBIOL & IMMUNOL LONDON N6A 3K7 ONTARIO CANADA
Titolo Testata:
Neuroscience
fascicolo: 1, volume: 56, anno: 1993,
pagine: 227 - 240
SICI:
0306-4522(1993)56:1<227:IORSPN>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPERIOR CERVICAL-GANGLION; SPINAL-CORD; PSEUDORABIES VIRUS; TRANSNEURONAL TRANSPORT; SYNAPTIC INPUT; NERVOUS-SYSTEM; ADRENAL-GLAND; BRAIN-STEM; NERVES; MEDULLA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
N.S. Dehal et al., "IDENTIFICATION OF RENAL SYMPATHETIC PREGANGLIONIC NEURONS IN HAMSTERSUSING TRANSSYNAPTIC TRANSPORT OF HERPES-SIMPLEX TYPE-1 VIRUS", Neuroscience, 56(1), 1993, pp. 227-240

Abstract

Herpes viruses have been used as retrograde transsynaptic tracers to identify pathways from the CNS to specific target tissues. We used herpes simplex virus to identify central nervous system neurons responsible for control of the kidney. Herpes simplex type 1 or herpes simplex type 2 was injected into rat kidneys and herpes simplex type 1 was microinjected into hamster and guinea pig kidneys. After three to seven days, ganglia, spinal cords and brains were examined using immunohistochemistry to visualize the virus-infected neurons. Our first experiments demonstrated that rats were not susceptible to infection with neurotropic strains of herpes simplex type 1. Injections of a wildtype strain of herpes simplex type 2 into rat kidneys led to nonspecific infection of many central nervous system neurons and glia. In contrast, herpes simplex type 1 injections in hamsters and guinea pigs caused specific infection of limited numbers of neurons in approximately one-third of the animals and the study was continued using hamsters. Sympathetic preganglionic neuron labelling was found in the ipsilateral intermediolateral cell column of the spinal cord as well as the lateral funiculus. Most infected preganglionic neurons were located in the seventh to the ninth thoracic spinal segments. Infected neurons were not found inthe dorsal or ventral horn of the spinal gray matter and only one or two cells were found in the brainstem. Sympathetic preganglionic neuron morphology was usually normal, showing detailed dendritic arborizations, and lysis was infrequent. Small infected cells were sometimes observed close to sympathetic preganglionic neurons. Because herpes simplex type 1 virus was not detected immunocytochemically in ganglionic neurons in these same hamsters, the polymerase chain reaction was used in some additional hamsters to detect viral DNA in the T12 and T13 chain ganglia and splanchnic ganglia ipsilateral to the kidney injected with herpes simplex type 1. Finally, the overall distribution of renal postganglionic and splanchnic preganglionic neurons in hamsters was examined for comparison to the number and locations of virus-labelled neurons. Retrograde transport of the fluorescent dye FluoroGold demonstrated that (i) renal postganglionic neurons are distributed in the T10-L1 chain ganglia and in the prevertebral splanchnic ganglion and (ii) splanchnic preganglionic neurons are located in the T3-T12 spinal segments, predominantly in the intermediolateral and funicular spinal autonomic nuclei. In conclusion, herpes simplex type 1 virus infected an exclusive population of ''renal'' neurons in hamsters without lysis and with little cellular reaction to the infection after a survival periodof three days, permitting these neurons to be studied in detail.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 23:18:22