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Titolo:
THE EFFECT OF BACULOVIRUS INFECTION ON ECDYSTEROID TITER IN GYPSY-MOTH LARVAE (LYMANTRIA-DISPAR)
Autore:
PARK EJ; BURAND JP; YIN CM;
Indirizzi:
UNIV MASSACHUSETTS,DEPT MICROBIOL AMHERST MA 01003 UNIV MASSACHUSETTS,DEPT MICROBIOL AMHERST MA 01003 UNIV MASSACHUSETTS,DEPT ENTOMOL AMHERST MA 01003
Titolo Testata:
Journal of insect physiology
fascicolo: 9, volume: 39, anno: 1993,
pagine: 791 - 796
SICI:
0022-1910(1993)39:9<791:TEOBIO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR POLYHEDROSIS-VIRUS; MANDUCA-SEXTA; GENE; METABOLISM;
Keywords:
GYPSY MOTH; BACULOVIRUS; LYMANTRIA-DISPAR; NUCLEAR POLYHEDROSIS VIRUS; LDNPV; ECDYSONE; ECDYSTEROID UDP GLUCOSYL TRANSFERASE; HEMOLYMPH ECDYSTEROID TITER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
E.J. Park et al., "THE EFFECT OF BACULOVIRUS INFECTION ON ECDYSTEROID TITER IN GYPSY-MOTH LARVAE (LYMANTRIA-DISPAR)", Journal of insect physiology, 39(9), 1993, pp. 791-796

Abstract

Insect baculovirus carries a gene referred to as egt. This gene encodes an enzyme known as ecdysteroid UDP-glucosyl transferase which catalyzes the sugar conjugation of ecdysteroids. Using a gypsy moth embryonic cell line EGT activity of Lymantria dispar nuclear polyhedrosis virus was identified. The effect of egt gene expression following virus infection in gypsy moth larvae was studied by determining the ecdysteroid titer in hemolymph. A radioimmunoassay (RIA) was used to determine the ecdysteroid titer and TLC coupled with RIA was used to estimate levels of conjugated and unconjugated ecdysteroids. Both the level and pattern of hemolymph ecdysteroid titer in virus-infected larvae are different from uninfected control insects. The hemolymph ecdysteroid titer of virus-infected insects never rise above basal level during larvalto pupal molting. However, during fourth to fifth larval molting, thehemolymph of virus-infected larvae shows higher levels of ecdysteroids than controls. In these insects the proportion of the sugar conjugated form of ecdysone was found to increase as virus replication proceeded.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:51:11