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Titolo:
THE SOLUBLE POOL OF BETA-2-MICROGLOBULIN FREE HLA CLASS-I ALPHA-CHAINS - QUALITATIVE AND QUANTITATIVE CHARACTERIZATION
Autore:
PICKL WF; MAJDIC O; FAE I; REUSCHEL R; HOLTER W; KNAPP W;
Indirizzi:
UNIV VIENNA,INST IMMUNOL,BORSCHKEGASSE 8A A-1090 VIENNA AUSTRIA UNIV VIENNA,INST IMMUNOL,BORSCHKEGASSE 8A A-1090 VIENNA AUSTRIA UNIV VIENNA,INST BLOOD GRP SEROL A-1090 VIENNA AUSTRIA SANDOZ GMBH A-1235 VIENNA AUSTRIA
Titolo Testata:
The Journal of immunology
fascicolo: 5, volume: 151, anno: 1993,
pagine: 2613 - 2622
SICI:
0022-1767(1993)151:5<2613:TSPOBF>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY ANTIGENS; T-CELL; LYMPHOCYTES-T; HEAVY-CHAINS; MOLECULES; MHC; SURFACE; VIRUS; RESTRICTION; ASSOCIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
W.F. Pickl et al., "THE SOLUBLE POOL OF BETA-2-MICROGLOBULIN FREE HLA CLASS-I ALPHA-CHAINS - QUALITATIVE AND QUANTITATIVE CHARACTERIZATION", The Journal of immunology, 151(5), 1993, pp. 2613-2622

Abstract

Previous studies have demonstrated that HLA class I heterodimers are present in plasma and cell culture supernatants. They can be precipitated by mAb the binding of which is dependent on the proper associationof the polymorphic alpha-chain with beta2-Microglobulin (beta2-m). The molecular mass of the alpha-chain ranges from 45 to 35 kDa with a number of intermediate products. We report on the identification of 35-kDa soluble beta2-M free HLA class I H chains immunoprecipitated by mAbLA45 from cell culture media of activated B and T cells. Furthermore,a peptide-based competitive immunosorbent assay was established to determine the amounts of soluble HLA class I alpha-chains. By means of this assay, we formally proved the specificity of mAb LA45 for a linearepitope on HLA class I H chains centered on residues arginine-asparagine at positions 62 and 63 of the alpha1-domain. PHA or rIL-2 were identified as efficient stimuli for PBMC leading to the generation of soluble beta2-m free HLA class I H chains. Testing of cell lines representing distinct stages of hematopoietic differentiation demonstrated a significant correlation between cell surface expression of beta2-m freeHLA class I H chains and amounts of soluble LA45 reactive molecules. However, three of six human T lymphotropic virus type I transfected cell lines, although expressing beta2-m free H chains, do not generate soluble molecules. Finally, human sera were found to contain considerable amounts of beta2-M free HLA class I H chains. The average amount ofthese molecules in sera of individuals with one positive LA45 allele was determined to be 46.9 +/- 38.6 nM/liter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:44:50