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Titolo:
EXPRESSION OF THE PASTEURELLA-HAEMOLYTICA LEUKOTOXIN IS INHIBITED BY A LOCUS THAT ENCODES AN ATP-BINDING CASSETTE HOMOLOG
Autore:
HIGHLANDER SK; WICKERSHAM EA; GARZA O; WEINSTOCK GM;
Indirizzi:
BAYLOR COLL MED,DEPT MICROBIOL & IMMUNOL,1 BAYLOR PLAZA HOUSTON TX 77030 UNIV TEXAS,SCH MED,DEPT BIOCHEM & MOLEC BIOL HOUSTON TX 77030
Titolo Testata:
Infection and immunity
fascicolo: 9, volume: 61, anno: 1993,
pagine: 3942 - 3951
SICI:
0019-9567(1993)61:9<3942:EOTPLI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI K-12; NUCLEOTIDE-SEQUENCE; EXTENSIVE HOMOLOGY; MALK PROTEIN; GENE; HEMOLYSIN; CLONING; IDENTIFICATION; TRANSPORT; PASTEURELLA-HAEMOLYTICA-A1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
S.K. Highlander et al., "EXPRESSION OF THE PASTEURELLA-HAEMOLYTICA LEUKOTOXIN IS INHIBITED BY A LOCUS THAT ENCODES AN ATP-BINDING CASSETTE HOMOLOG", Infection and immunity, 61(9), 1993, pp. 3942-3951

Abstract

Multicopy and single-copy chromosomal fusions between the Pasteurellahaemolytica leukotoxin regulatory region and the Escherichia coli beta-galactosidase gene have been constructed. These fusions were used asreporters to identify and isolate regulators of leukotoxin expressionfrom a P. haemolytica cosmid library. A cosmid clone, which inhibitedleukotoxin expression from multicopy and single-copy protein fusions,was isolated and found to contain the complete leukotoxin gene cluster plus additional upstream sequences. The locus responsible for inhibition of expression from leukotoxin-beta-galactosidase fusions was mapped within these upstream sequences, by transposon mutagenesis with Tn5, and its DNA sequence was determined. The inhibitory activity was found to be associated with a predicted 440-amino-acid reading frame (lapA) that lies within a four-gene arginine transport locus. LapA is predicted to be the nucleotide-binding component of this transport system and shares homology with the Clp family of proteases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 13:40:59