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Titolo:
TRANSCRIPTIONAL AND TRANSLATIONAL STUDY OF THE DROSOPHILA-SUBOBSCURA HSP83 GENE IN NORMAL AND HEAT-SHOCK CONDITIONS
Autore:
ARBONA M; DEFRUTOS R; TANGUAY RM;
Indirizzi:
UNIV VALENCIA,DEPT GENET,DR MOLINER 50 E-46100 VALENCIA SPAIN CHU LAVAL,CTR RECH ST FOY PQ CANADA
Titolo Testata:
Genome
fascicolo: 4, volume: 36, anno: 1993,
pagine: 694 - 700
SICI:
0831-2796(1993)36:4<694:TATSOT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHROMOSOMAL-POLYMORPHISM; DEVELOPMENTAL REGULATION; PROTEIN-SYNTHESIS; EXPRESSION; PATTERNS; LOCALIZATION;
Keywords:
DROSOPHILA-SUBOBSCURA; HSP83; HEAT-SHOCK GENES; IMMUNOBLOTTING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
M. Arbona et al., "TRANSCRIPTIONAL AND TRANSLATIONAL STUDY OF THE DROSOPHILA-SUBOBSCURA HSP83 GENE IN NORMAL AND HEAT-SHOCK CONDITIONS", Genome, 36(4), 1993, pp. 694-700

Abstract

In this paper we report a transcriptional and translational study of the hsp83 gene of Drosophila subobscura. This gene is located at the 18C region of the J chromosome. A monoclonal antibody raised against hsp83 was used for the immunological detection of this protein by Western blotting throughout the development of D. subobscura in control and heat-shock conditions. Our results indicate that puff 18C is not only heat-shock inducible but is also expressed during normal development and its level of expression increases at the end of the prepupa period. We detected hsp83 at normal temperatures, in particular developmentalstages with the exception of the larval and the beginning of prepupa formation. Hsp83 was induced by heat shock in all stages studied with the exception of 1st instar larvae. We found that temperatures in excess of 26-degrees-C were sufficient to induce hsp83. In addition, at temperatures from 26 to 34-degrees-C, the increase in hsp83 synthesis was accompanied by increased transcription of the hsp83 gene; this positive correlation was not observed at 37-degrees-C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 20:42:13