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Titolo:
IDENTIFICATION AND OLIGOSACCHARIDE STRUCTURE-ANALYSIS OF RHODOPSIN GLYCOFORMS CONTAINING GALACTOSE AND SIALIC-ACID
Autore:
DUFFIN KL; LANGE GW; WELPLY JK; FLORMAN R; OBRIEN PJ; DELL A; REASON AJ; MORRIS HR; FLIESLER SJ;
Indirizzi:
EYE INST,1755 S GRAND BLVD ST LOUIS MO 63104 EYE INST,1755 S GRAND BLVD ST LOUIS MO 63104 MONSANTO CO,RES ST LOUIS MO 63198 NIH BETHESDA MD 20892 UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM LONDON SW7 2AZ ENGLAND ST LOUIS UNIV,SCH MED,EA DOISY DEPT BIOCHEM & MOLEC BIOL ST LOUIS MO 63104
Titolo Testata:
Glycobiology
fascicolo: 4, volume: 3, anno: 1993,
pagine: 365 - 380
SICI:
0959-6658(1993)3:4<365:IAOSOR>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER SEGMENT MEMBRANES; N-LINKED OLIGOSACCHARIDES; LECTIN BINDING-SITES; PHOTORECEPTOR CELLS; ULTRASTRUCTURAL-LOCALIZATION; RETINAL PHOTORECEPTORS; PIGMENT-EPITHELIUM; MASS-SPECTROMETRY; BOVINE RHODOPSIN; RECEPTORS;
Keywords:
GLYCOFORM; OLIGOSACCHARIDE STRUCTURE; RHODOPSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
K.L. Duffin et al., "IDENTIFICATION AND OLIGOSACCHARIDE STRUCTURE-ANALYSIS OF RHODOPSIN GLYCOFORMS CONTAINING GALACTOSE AND SIALIC-ACID", Glycobiology, 3(4), 1993, pp. 365-380

Abstract

The N-linked oligosaccharides of frog (Rana pipiens) rhodopsin were analysed by sequential exoglycosidase digestion and gel filtration chromatography, following reductive tritiation. In addition, selected tryptic glycopeptides obtained from frog retinal rod outer segment membranes were examined by electrospray mass spectrometry (ES-MS), fast atom bombardment mass spectrometry (FAB-MS), amino acid sequence and composition analysis, and carbohydrate composition analysis. The amino acid sequence data demonstrated that the glycopeptides were derived from rhodopsin and confirmed the presence of two N-glycosylation sites, at residues Asn2 and Asn15. The predominant glycan (approximately 60% of total) had the structure GlcNAcbeta1-2Manalpha1-3(Manalpha1-6) Manbeta1-4GlcNAcbeta1-4GlcNAc-(Asn), with the remaining structures containing 1-3 additional hexose residues, as reported previously for bovine rhodopsin. Unlike bovine rhodopsin, however, a sizable fraction of the total glycans of frog rhodopsin also contained sialic acid (NeuAc), with the sialylated oligosaccharides being present exclusively at the Asn2 site. FAB-MS analysis of oligosaccharides released from the Asn2 site gave, among other signals, an abundant quasimolecular ion correspondingto a glycan of composition NeuAc1Hex6HexNAc3 (where Hex is hexose andHexNAc is N-acetylhexosamine), consistent with a hybrid structure. The potential biological implications of these results are discussed in the context of rod outer segment membrane renewal.

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Documento generato il 28/09/20 alle ore 15:23:13