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Titolo:
DEXAMETHASONE STIMULATES RIBOSOMAL-RNA GENE-TRANSCRIPTION IN RAT MYOBLASTS
Autore:
GLIBETIC M; LARSON DE; ROTHBLUM LI; SELLS BH;
Indirizzi:
UNIV GUELPH,COLL BIOL SCI,DEANS OFF GUELPH N1G 2W1 ONTARIO CANADA UNIV GUELPH,DEPT MOLEC BIOL & GENET GUELPH N1G 2W1 ONTARIO CANADA GEISINGER MED CLIN,SIGFRIED & JANET WEIS CTR RES DANVILLE PA 00000
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 2, volume: 94, anno: 1993,
pagine: 243 - 252
SICI:
0303-7207(1993)94:2<243:DSRGIR>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA SYNTHESIS; POLYMERASE-I TRANSCRIPTION; TATA-BINDING PROTEIN; LYMPHOSARCOMA P1798 CELLS; FACTOR TIF-IA; HORMONAL-REGULATION; MESSENGER-RNA; RECEPTOR-BINDING; MUSCLE-CELLS; MOUSE RDNA;
Keywords:
MYOBLASTS; RIBOSOMAL-RNA GENE EXPRESSION; DEXAMETHASONE; RU38486; UBF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
M. Glibetic et al., "DEXAMETHASONE STIMULATES RIBOSOMAL-RNA GENE-TRANSCRIPTION IN RAT MYOBLASTS", Molecular and cellular endocrinology, 94(2), 1993, pp. 243-252

Abstract

The glucocorticoid analogue, dexamethasone, stimulated RNA synthesis more than two-fold in rat L6 myoblasts, without affecting the rate of cell proliferation. Treatment of myoblasts for 24 h with 10(-7) M dexamethasone resulted in a 30% increase in the cellular RNA level. More than a two-fold stimulation of pre-rRNA gene transcription by dexamethasone, as measured in isolated nuclei and by cell-free transcription, was accompanied by a corresponding increase in pre-rRNA levels. Co-incubation of myoblasts with cycloheximide and dexamethasone did not affect the enhanced pre-rRNA gene transcription demonstrating that de novo protein synthesis was unnecessary to manifest the dexamethasone effecton rDNA transcription. Support for this conclusion is provided by thefinding that the levels of UBF1 and UBF2, rDNA upstream binding transcription factors, remain unchanged. The glucocorticoid antagonist RU38486 hydroxy-17alpha-(prop-1-ynyl)estra-4,9-dien-3-one] inhibited the dexamethasone-stimulated rRNA gene transcription suggesting that the glucocorticoid receptor is involved in the response mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 18:07:21