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Titolo:
ANALYSIS OF GAMMA-AMINOBUTYRIC ACID(A) RECEPTOR SUBUNITS IN THE MOUSECOCHLEA BY MEANS OF THE POLYMERASE CHAIN-REACTION
Autore:
DRESCHER DG; GREEN GE; KHAN KM; HAJELA K; BEISEL KW; MORLEY BJ; GUPTA AK;
Indirizzi:
WAYNE STATE UNIV,SCH MED,DEPT OTOLARYNGOL,BIOOTOL LAB,540 E CANFIELD AVE DETROIT MI 48201 WAYNE STATE UNIV,SCH MED,DEPT BIOCHEM,BIOOTOL LAB DETROIT MI 48201 BOYS TOWN NATL INST COMMUN DISORDERS CHILDREN OMAHA NE 00000 AGA KHAN UNIV KARACHI PAKISTAN
Titolo Testata:
Journal of neurochemistry
fascicolo: 3, volume: 61, anno: 1993,
pagine: 1167 - 1170
SICI:
0022-3042(1993)61:3<1167:AOGARS>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GABA-A RECEPTOR; GUINEA-PIG COCHLEA; OUTER HAIR-CELLS; ALPHA-SUBUNIT; BENZODIAZEPINE RECEPTORS; FUNCTIONAL EXPRESSION; RAT; IMMUNOREACTIVITY; PHARMACOLOGY; SUBTYPES;
Keywords:
GABA BENZODIAZEPINE RECEPTORS; PCR; COCHLEA; GABA;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
D.G. Drescher et al., "ANALYSIS OF GAMMA-AMINOBUTYRIC ACID(A) RECEPTOR SUBUNITS IN THE MOUSECOCHLEA BY MEANS OF THE POLYMERASE CHAIN-REACTION", Journal of neurochemistry, 61(3), 1993, pp. 1167-1170

Abstract

Thirteen GABA(A) receptor subunits were examined in the cochlea of the CBA(J) mouse by PCR analysis. Total RNA was extracted from the auditory organs of 14-18-day-old animals, and mRNA was isolated using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with primers specific for nucleotide sequences representing GABA(A) subunits. PCR products corresponding to subunits alpha1-alpha6, beta1-beta3, and gamma2 were amplified, whereas those representing gamma, gamma3, and delta were not amplified above background. These results provide the most direct evidence to date that GABA(A) receptors composed of the detected subunits are expressed in the mammalian cochlea, lending new support to previous studies implicating GABA as a cochlear transmitter. The pronounced expression Of alpha2 and alpha6 subunits suggests type II and ''cerebellar-type'' benzodiazepine pharmacology in the cochlea.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 22:58:40