Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ECTOCELLULAR IN-VITRO AND IN-VIVO METABOLISM OF CADP-RIBOSE IN CEREBELLUM
Autore:
DEFLORA A; GUIDA L; FRANCO L; ZOCCHI E; PESTARINO M; USAI C; MARCHETTI C; FEDELE E; FONTANA G; RAITERI M;
Indirizzi:
UNIV GENOA,IST CHIM BIOL,VIA BENEDETTO XV-1 I-16132 GENOA ITALY CTR BIOTECNOL AVANZATE I-16132 GENOA ITALY UNIV GENOA,IST ANAT COMPARATA I-16132 GENOA ITALY CNR,IST CIBERNET & BIOFIS I-16149 GENOA ITALY UNIV GENOA,IST FARMACOL & FARMACOGNOSIA I-16148 GENOA ITALY
Titolo Testata:
Biochemical journal
, volume: 320, anno: 1996,
parte:, 2
pagine: 665 - 671
SICI:
0264-6021(1996)320:<665:EIAIMO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC ADP-RIBOSE; PANCREATIC BETA-CELLS; LEUKEMIC HL-60 CELLS; ANTIGEN CD38; CA2+ RELEASE; NAD GLYCOHYDROLASE; INTRACELLULAR CA2+; HUMAN ERYTHROCYTES; CYCLASE ACTIVITY; CALCIUM CURRENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
A. Deflora et al., "ECTOCELLULAR IN-VITRO AND IN-VIVO METABOLISM OF CADP-RIBOSE IN CEREBELLUM", Biochemical journal, 320, 1996, pp. 665-671

Abstract

CD38, a type II transmembrane glycoprotein predominantly expressed inblood cells, is a bifunctional ectoenzyme directly involved in the metabolism of cADP-ribose (cADPR). This is a potent Ca2+ mobilizer in several types of cells. The relationship between the ectocellular site of cADPR production and its intracellular calcium-related functions is poorly understood. Cultured rat cerebellar granule cells showed both enzymic activities of CD38, ADP-ribosyl cyclase and cADPR hydrolase, ata ratio of 16 to 1 respectively, and were immunostained by the anti (human CD38) monoclonal antibody IB4. In these cells externally added cADPR and beta-NAD(+) (the precursor of cADPR), but not alpha-NAD(+) orADP-ribose, enhanced the peak of the depolarization-induced rise in intracellular Ca2+ concentration. This effect was inhibited by 1 mu M ryanodine, suggesting a potentiation of calcium-induced calcium releaseby cADPR. CD38 ectoenzyme activities, ADP-ribosyl cyclase and cADPR hydrolase, were also demonstrated in viva by microdialysis of adult ratcerebellum, where IB4 bound to granule neurons selectively. Trace amounts (11.5+/-3.8 nM) of NAD(+) were detected by microdialysis samplingand sensitive assays in the basal interstitial fluid of the cerebellum. These results provide a link between ectocellular cADPR turnover and intracellular calcium mobilization in cerebellum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 14:17:55