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Titolo:
DETECTION OF 7 SPECIES OF PATHOGENIC LEPTOSPIRES BY PCR USING 2 SETS OF PRIMERS
Autore:
GRAVEKAMP C; VANDEKEMP H; FRANZEN M; CARRINGTON D; SCHOONE GJ; VANEYS GJJM; EVERARD COR; HARTSKEERL RA; TERPSTRA WJ;
Indirizzi:
WHO,FAO,COLLABORATING CTR REFERENCE & RES LEPTOSPIROSIS,ROYAL TROP INST 1105 AZ AMSTERDAM NETHERLANDS UNIV LIMBURG,DEPT MOLEC CELL BIOL 6200 MO MAASTRICHT NETHERLANDS GOVT BARBADOS,MRC,LEPTOSPIROSIS LAB ST MICHAEL BARBADOS
Titolo Testata:
Journal of General Microbiology
, volume: 139, anno: 1993,
parte:, 8
pagine: 1691 - 1700
SICI:
0022-1287(1993)139:<1691:DO7SOP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MYCOBACTERIUM-LEPRAE; GEL-ELECTROPHORESIS; DNA AMPLIFICATION; CLINICAL-SAMPLES; URINE; SEQUENCES; HYBRIDIZATION; SEROVARS; BLOOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
C. Gravekamp et al., "DETECTION OF 7 SPECIES OF PATHOGENIC LEPTOSPIRES BY PCR USING 2 SETS OF PRIMERS", Journal of General Microbiology, 139, 1993, pp. 1691-1700

Abstract

Two sets of primers derived from genomic DNA libraries of Leptospira serovars icterohaemorrhagiae (strain RGA) and bim (strain 1051)enabledthe amplification by PCR of target DNA fragments from leptospiral reference strains belonging to all presently described pathogenic Leptospira species. The icterohaemorrhagiae-derived primers (G1/G2) enabled amplification of DNA from L. interrogans, L. borgpetersenii, L. weilii,L. noguchii, L. santarosai and L. meyeri, whereas the bim-derived primers (B64-I/B64-II) enabled the amplification of L. kirschneri. Southern blot and DNA sequence analysis revealed inter-species DNA polymorphism within the region spanned by primers G1 and G2 between L. interrogans and various other Leptospira species. Using a mixture of primer sets G1/G2 and B64-I/B64-II, leptospires of serovars icterohaemorrhagiae, copenhageni, hardjo, pomona, grippotyphosa and bim were detected in serum samples collected from patients during the first 10 days after the onset of illness.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:12:50