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Titolo:
A METHOD FOR THE DETECTION OF ERYTHROCYTE-BOUND INTERLEUKIN-8 IN HUMANS DURING INTERLEUKIN-1 IMMUNOTHERAPY
Autore:
TILG H; PAPE D; TREHU E; SHAPIRO L; ATKINS MB; DINARELLO CA; MIER JW;
Indirizzi:
TUFTS UNIV NEW ENGLAND MED CTR,DEPT MED,DIV HEMATOL ONCOL,750 WASHINGTON ST,BOX 245 BOSTON MA 02111 TUFTS UNIV NEW ENGLAND MED CTR,DEPT MED,DIV HEMATOL ONCOL,750 WASHINGTON ST,BOX 245 BOSTON MA 02111 TUFTS UNIV NEW ENGLAND MED CTR,DEPT MED,DIV INFECT DIS BOSTON MA 02111 TUFTS UNIV,SCH MED BOSTON MA 02111
Titolo Testata:
Journal of immunological methods
fascicolo: 2, volume: 163, anno: 1993,
pagine: 253 - 258
SICI:
0022-1759(1993)163:2<253:AMFTDO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
IL-8; ENDOTOXIN; CELLS;
Keywords:
IL-8; ERYTHROCYTE-BOUND IL-8; IL-1-ALPHA, IMMUNOTHERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
H. Tilg et al., "A METHOD FOR THE DETECTION OF ERYTHROCYTE-BOUND INTERLEUKIN-8 IN HUMANS DURING INTERLEUKIN-1 IMMUNOTHERAPY", Journal of immunological methods, 163(2), 1993, pp. 253-258

Abstract

Interleukin-8 (IL-8) has been recently shown to bind to human erythrocytes with high affinity and is therefore potentially difficult to detect in serum or plasma. IL-8 is transiently elevated in the serum of baboons after the administration of interleukin-1alpha (IL-1alpha). Theobjective of this study was to investigate whether IL-8 can be detected in the plasma or in detergent-lysed erythrocytes from cancer patients undergoing treatment with IL-1alpha. Using a specific radioimmunoassay (RIA), plasma IL-8 was detected within 1-2 h after the first IL-1alpha infusion. Thereafter, the levels declined rapidly and after 4-8 hwere undetectable. Erythrocyte-bound IL-8 was detectable 1-2 h after the increase in plasma levels. The erythrocyte-bound IL-8 levels were higher than those measured in plasma and remained elevated long after the plasma levels had become undetectable. Erythrocyte membranes accounted for all of the erythrocyte-associated IL-8, as IL-8 was undetectable in the cytosol after erythrocyte lysis. The assay used in these studies detects IL-8 in erythrocyte lysates when it cannot be measured in plasma and may therefore be useful in monitoring IL-8 production in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:24:39