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Titolo:
EXPANSION OF CORD-BLOOD PROGENITORS AND USE FOR HEMATOPOIETIC RECONSTITUTION
Autore:
GABUTTI V; TIMEUS F; RAMENGHI U; CRESCENZIO N; MARRANCA D; MINIERO R; CORNAGLIA G; BAGNARA GP;
Indirizzi:
UNIV TURIN,DEPT PEDIAT,PIAZZA G C DOGLIOTTI 94 I-10126 TURIN ITALY UNIV TURIN,DEPT GYNECOL & OBSTET I-10126 TURIN ITALY UNIV BOLOGNA,INTERDEPT CTR CANC RES I-40126 BOLOGNA ITALY
Titolo Testata:
Stem cells
, volume: 11, anno: 1993, supplemento:, 2
pagine: 105 - 112
SICI:
1066-5099(1993)11:<105:EOCPAU>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-KIT LIGAND; HEMATOPOIETIC PROGENITORS; BONE-MARROW; FANCONIS ANEMIA; CELLS; GROWTH; TRANSPLANTATION; INTERLEUKIN-6; PRECURSORS; TERM;
Keywords:
HUMAN CORD BLOOD; HEMATOPOIETIC PROGENITORS; CELL PROLIFERATION; CYTOKINES; STEM CELL EXPANSION; TRANSPLANTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
V. Gabutti et al., "EXPANSION OF CORD-BLOOD PROGENITORS AND USE FOR HEMATOPOIETIC RECONSTITUTION", Stem cells, 11, 1993, pp. 105-112

Abstract

A high number of stem cells migrate in fetal blood and, at birth, thenumber of progenitors in cord blood equals or exceeds that of adult bone marrow. Recently hemopoiesis has been successfully reconstituted with the infusion of cord blood cells. It is important to clearly define the quantity and quality of cord blood totipotent and multilineage progenitors to evaluate the possibility of their utilization in transplants. Our first aim was to study the growth characteristics of cord blood progenitors. We have evaluated the number of cycling cells with the thymidine suicide technique and the production, by phytohemagglutinin (PHA) stimulated cord blood mononuclear cells, of some cytokines involved in the proliferation of progenitor cells, such as granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin 6 (IL-6) and leukemia inhibitory factor (LIF). We have also studied by flow cytometry the CD34+CD33-, CD34+CD33, cell subsets and the presence of the c-kit receptor in order to quantitate the number of earlier progenitors. Our second aim was to elucidate whether the cord blood totipotent stemcell population or the committed progenitors could be expanded in vitro. Our results showed that in cord blood the number of early progenitors, as evaluated by the number of mixed lineage colony forming units (CFU-Mix), by the CD34+CD33- subsets and the expression of the c-kit, is higher than in bone marrow. We have also demonstrated the possibility in vitro of increasing the number of progenitors by more than 30-fold by utilizing stem cell factor (SCF) in association with other cytokines. These findings may be relevant for transplant practice since they offer the means to enhance hematopoietic recovery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 15:53:56