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Titolo:
EFFECTS OF BETA-ADRENERGIC-RECEPTOR ACTIVATION ON ALVEOLAR MACROPHAGECYTOPLASMIC MOTILITY
Autore:
FUKUSHIMA T; SEKIZAWA K; JIN Y; YAMAYA M; SASAKI H; TAKISHIMA T;
Indirizzi:
TOHOKU UNIV,SCH MED,DEPT INTERNAL MED 1,1-1 SEIRYOMACHI,AOBA KU SENDAI MIYAGI 980 JAPAN TOHOKU UNIV,SCH MED,DEPT INTERNAL MED 1,1-1 SEIRYOMACHI,AOBA KU SENDAI MIYAGI 980 JAPAN
Titolo Testata:
The American journal of physiology
fascicolo: 1, volume: 265, anno: 1993,
parte:, 1
pagine: 120000067 - 120000072
SICI:
0002-9513(1993)265:1<120000067:EOBAOA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ADENYLATE-CYCLASE; MAGNETIC PARTICLES; CHOLERA-TOXIN; DUAL CONTROL; CYCLIC-AMP; MECHANISMS; CELLS; LUNGS;
Keywords:
REMANENT FIELD STRENGTH; CHOLERA TOXIN-SENSITIVE G-PROTEINS; ADENOSINE 3',5'-CYCLIC MONOPHOSPHATE; PROTEIN KINASE-A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
T. Fukushima et al., "EFFECTS OF BETA-ADRENERGIC-RECEPTOR ACTIVATION ON ALVEOLAR MACROPHAGECYTOPLASMIC MOTILITY", The American journal of physiology, 265(1), 1993, pp. 120000067-120000072

Abstract

We studied the effects of fenoterol, a beta-adrenoceptor agonist, on the cytoplasmic motility of alveolar macrophages (AM) from dog lungs in vitro. Four days after the instillation of Fe3O4 particles (3 mg/kg)into the lower lobe bronchus, AM were harvested by bronchoalveolar lavage. Remanent field strength (RFS) from the AM containing Fe3O4 particles (5 X 10(6) cells) was measured immediately after magnetization. RFS decreased with time due to particle rotation (relaxation), which isrelated to cytoplasmic motility of AM. Fenoterol (10(-9) M to 10(-5) M) decreased the relaxation rate (lambda0; min-1) in a concentration-dependent fashion with the maximum effect at 10(-6) M. Both forskolin (10(-6) M to 10(-4) M) and dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP) (10(-3) M) mimicked fenoterol-induced inhibitory effects on lambda0. Fenoterol and forskolin concentration-dependently increased intracellular levels of cAMP, which were parallel to decreases in lambda0 induced by these drugs. KT 5720 (10(-5) M), a specific inhibitor ofprotein kinase A, significantly inhibited fenoterol (10(-6) M)-induced inhibitory effects on lambda0 (P < 0.01). These results imply that beta-adrenergic receptor activation inhibits cytoplasmic motility of AMvia increases in intracellular levels of cAMP, which may be coupled with activation of a cAMP-dependent protein kinase.

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Documento generato il 03/08/20 alle ore 13:02:04