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Titolo:
MECHANISM OF THE PROLACTIN REBOUND AFTER DOPAMINE WITHDRAWAL IN RAT PITUITARY-CELLS
Autore:
CHEN C; ZHANG J; ISRAEL JM; CLARKE IJ; VINCENT JD;
Indirizzi:
PRINCE HENRYS INST MED RES,POB 152 CLAYTON VIC 3168 AUSTRALIA INSERM,U176 F-33077 BORDEAUX FRANCE
Titolo Testata:
The American journal of physiology
fascicolo: 1, volume: 265, anno: 1993,
parte:, 1
pagine: 50000145 - 50000152
SICI:
0002-9513(1993)265:1<50000145:MOTPRA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYROTROPIN-RELEASING-HORMONE; DEPENDENT CALCIUM CURRENTS; ENRICHED PRIMARY CULTURES; ANTERIOR-PITUITARY; ELECTROPHYSIOLOGICAL RESPONSES; ADENYLATE-CYCLASE; HYPOPHYSEAL CELLS; LACTOTROPH CELLS; LACTATING RATS; CYCLIC-AMP;
Keywords:
PITUITARY; ADENOSINE 3',5'-CYCLIC MONOPHOSPHATE; THYROTROPIN-RELEASING HORMONE; D(2)-RECEPTOR; G-PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
C. Chen et al., "MECHANISM OF THE PROLACTIN REBOUND AFTER DOPAMINE WITHDRAWAL IN RAT PITUITARY-CELLS", The American journal of physiology, 265(1), 1993, pp. 50000145-50000152

Abstract

To study the mechanism underlying the effect of dopamine withdrawal on prolactin release, continuous perifusion experiments were performed on rat lactotroph-enriched primary cultures. Removal of dopamine (10(-7) M) after a short-term application (15 min) produced a rebound of prolactin secretion, which was enhanced by pretreatment of the cell culture with 17beta-estradiol (10(-8) M for 48 h). Ca2+ channel blockade by Co2+ (1 mM) abolished the rebound in prolactin release. An increase in intracellular adenosine 3',5'-cyclic monophosphate by either forskolin (5 muM) or 3-isobutyl-1-methylxanthine (100 muM) enhanced the prolactin rebound after dopamine withdrawal. Application of thyrotropin-releasing hormone (10-7 M) increased the prolactin rebound after dopamine withdrawal with a maximum effect obtained by commencing treatment immediately after removal of dopamine. Pretreatment of cell cultures with pertussis toxin (100 ng/ml, for 10 h) totally abolished the effects of dopamine on prolactin secretion. The dopamine agonist bromocriptine(10(-9) M) significantly decreased prolactin secretion, but no rebound effect was observed after its removal. We conclude that the rebound of prolactin release after dopamine treatment involves the influx of Ca2+.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/20 alle ore 20:18:26