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Titolo:
EFFECTS OF EXTRACELLULAR ATP ON ION-TRANSPORT AND [CA2-EAR EPITHELIUM(](I) IN MONGOLIAN GERBIL MIDDLE)
Autore:
FURUKAWA M; IKEDA K; YAMAYA M; OSHIMA T; SASAKI H; TAKASAKA T;
Indirizzi:
TOHOKU UNIV,SCH MED,DEPT OTORHINOLARYNGOL,AOBA KU,1-1 SEIRYO MACHI SENDAI MIYAGI 98077 JAPAN TOHOKU UNIV,SCH MED,DEPT GERIATR MED,AOBA KU SENDAI MIYAGI 98077 JAPAN
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 3, volume: 41, anno: 1997,
pagine: 827 - 836
SICI:
0363-6143(1997)41:3<827:EOEAOI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECTIFYING CHLORIDE CHANNELS; CYSTIC-FIBROSIS; ELECTROLYTE TRANSPORT; INTRACELLULAR CALCIUM; TRACHEAL EPITHELIUM; POTASSIUM CHANNELS; PRIMARY CULTURES; OTITIS-MEDIA; CELL-LINE; SECRETION;
Keywords:
CELL CULTURE; CHLORIDE SECRETION; SHORT-CIRCUIT CURRENT; USSING CHAMBERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
M. Furukawa et al., "EFFECTS OF EXTRACELLULAR ATP ON ION-TRANSPORT AND [CA2-EAR EPITHELIUM(](I) IN MONGOLIAN GERBIL MIDDLE)", American journal of physiology. Cell physiology, 41(3), 1997, pp. 827-836

Abstract

The present study investigates the effects of ATP on ion transport and Ca2+ mobilization in primary cultures of gerbil middle ear epithelium. Extracellular ATP and other nucleotides elicited a transient increase in short-circuit current (I-sc) in a dose-dependent manner. The rank order of potency in I-sc responses corresponded with the sequence ofthe P-2u purinergic gic receptor when applied to the mucosal or the basolateral side. Extracellular ATP induced a rise in the intracellularCa2+ concentration ([Ca2+](i)) in a dose-dependent manner. A similar rank order of potency in the ATP-induced increases in [Ca2+](i) was observed. The time courses of the [Ca2+](i) responses induced by ATP closely paralleled those of the I-sc responses. 2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetra-acetic acid acetoxymethyl ester greatly reduced theincrease in the I-sc and [Ca2+](i) responses to ATP. Diphenylamine-2-carboxylate, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, quinidine, and Ba2+ significantly blocked the ATP-stimulated I-sc. Glibenclamide had no effect on the ATP-stimulated I-sc. These results are consistent with the hypothesis that extracellular ATP and other nucleotides induce Cl- secretion across the apical membrane in cultured gerbil middle ear epithelium by activation of the apical Cl- channels via multiple modes of regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 14:37:29