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Titolo:
THE NUMBER OF GLOMERULAR CAPILLARIES ESTIMATED BY AN UNBIASED AND EFFICIENT STEREOLOGICAL METHOD
Autore:
NYENGAARD JR; MARCUSSEN N;
Indirizzi:
AARHUS UNIV,INST PATHOL,STEREOLOG RES LAB DK-8000 AARHUS DENMARK AARHUS UNIV,INST EXPTL CLIN RES,INTERNAL MED CLIN 2 DK-8000 AARHUS DENMARK
Titolo Testata:
Journal of Microscopy
, volume: 171, anno: 1993,
parte:, 1
pagine: 27 - 37
SICI:
0022-2720(1993)171:<27:TNOGCE>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARBITRARY PARTICLES; RAT; LENGTH; HYPERTROPHY; SECTIONS; DENSITY; SURFACE; NETWORK; NEURONS; PATTERN;
Keywords:
CAPILLARY NUMBER; CONNECTIVITY; DISSECTOR; EULER-POINCARE CHARACTERISTIC; GLOMERULUS; ISOTROPY; NETWORKS; SAMPLING EFFICIENCY; STEREOLOGY; TOPOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
J.R. Nyengaard e N. Marcussen, "THE NUMBER OF GLOMERULAR CAPILLARIES ESTIMATED BY AN UNBIASED AND EFFICIENT STEREOLOGICAL METHOD", Journal of Microscopy, 171, 1993, pp. 27-37

Abstract

A method for unbiased capillary number estimation based on the estimation of the Euler-Poincare characteristic or Euler number with the disector is evaluated. The generation of a new capillary creates a new loop in the capillary network corresponding to a change of exactly one unit in the Euler number of the network. In this way the Euler number has a one-to-one relationship to the number of capillaries in a network. In this report rat glomerular capillaries are counted. Three tissue blocks from each of 14 perfusion-fixed rat kidneys, aged between 5 days and 18 months, were uniformly sampled and embedded in Epon. Two complete glomerular profiles were sampled per block. Three consecutive sections were studied per glomerulus using the middle one to evaluate thetopological events of the capillaries in the sampled glomerulus. The use of complete glomerular profiles eliminated problems with the edges, whereas the disector ensured unbiased sampling in the third dimension. Estimates per animal were weighted averages over blocks, the weights being half the number of glomerular profiles in a section from a block. The number of capillaries in a glomerulus is the mean Euler numberper disector volume multiplied by the mean volume of glomeruli obtained by the fractionator. The observed coefficient of variation between animals was 18.3% for the estimated number of capillaries per glomerulus, and the observed coefficient of error at the level of blocks within animals was 14.7%. The exact capillary number from the node-branch network of two reconstructed glomeruli equalled the capillary number obtained by the total Euler number from the same glomeruli. This shows in an applied example, as would be expected, that the estimation of capillary number using the Euler number is unbiased and independent of the direction of sectioning.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 11:35:01