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Titolo:
IDENTIFICATION OF THE MCRC GENE-PRODUCT IN METHANOCOCCUS-VANNIELII
Autore:
STROUP D; REEVE JN;
Indirizzi:
OHIO STATE UNIV,DEPT MICROBIOL COLUMBUS OH 43210 OHIO STATE UNIV,DEPT MICROBIOL COLUMBUS OH 43210
Titolo Testata:
FEMS microbiology letters
fascicolo: 1, volume: 111, anno: 1993,
pagine: 129 - 134
SICI:
0378-1097(1993)111:1<129:IOTMGI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
COENZYME-M REDUCTASE; BACTERIOPHAGE-T7 RNA-POLYMERASE; COMPONENT-C; EXPRESSION; METHANOBACTERIUM; METHANOGENS; SYSTEM;
Keywords:
METHANOGEN; METHYL REDUCTASE; MCRC GENE PRODUCT; METHANOCOCCUS-VANNIELII;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
D. Stroup e J.N. Reeve, "IDENTIFICATION OF THE MCRC GENE-PRODUCT IN METHANOCOCCUS-VANNIELII", FEMS microbiology letters, 111(1), 1993, pp. 129-134

Abstract

The polypeptide encoded by the mcrC gene has been identified in Methanococcus vannielii by immunoblotting using rabbit antibodies raised against the product of a lacZ-mcrC gene fusion synthesized and purified from Escherichia coli. The mcrC gene product (gpmcrC) was located in both the supernatant and pellet fractions after centrifugation of Mc. vannielii cell extracts for 2 h at 100000 X g. When anaerobic reducing conditions were maintained during purification, gpmcrC co-sedimented through sucrose gradients to the same position as molecules of the methyl coenzyme M reductase holoenzyme (approx. 300 kDa). This co-sedimentation was lost under aerobic, nonreducing conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:07:26