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Titolo:
EFFECT OF SERA FROM MYASTHENIA-GRAVIS PATIENTS AND OF ALPHA-BUNGAROTOXIN ON ACETYLCHOLINESTERASE DURING IN-VITRO NEUROMUSCULAR SYNAPTOGENESIS
Autore:
DELAPORTE S; RAGUEH F; EYMARD B; COURBIN P; CHAPRON J; KOENIG J;
Indirizzi:
UNIV BORDEAUX 2,NEUROBIOL CELLULAIRE LAB,CNRS,URA 1126,AVE FAC F-33405 TALENCE FRANCE CTR CHIRURG MARIE LANNELONGUE,IMMUNOL LAB,CNRS,URA 1159 F-92350 LE PLESSIS ROBINS FRANCE
Titolo Testata:
Journal of the neurological sciences
fascicolo: 1-2, volume: 117, anno: 1993,
pagine: 92 - 102
SICI:
0022-510X(1993)117:1-2<92:EOSFMP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE-MUSCLE CULTURES; SKELETAL-MUSCLE; MOLECULAR-FORMS; CLINICAL STATE; RAT MYOTUBES; BASAL LAMINA; SPINAL-CORD; RECEPTOR; CELLS; JUNCTION;
Keywords:
NERVE-MUSCLE CULTURES; MYASTHENIA GRAVIS; ALPHA-BUNGAROTOXIN; ACETYLCHOLINESTERASE; ACETYLCHOLINE RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
S. Delaporte et al., "EFFECT OF SERA FROM MYASTHENIA-GRAVIS PATIENTS AND OF ALPHA-BUNGAROTOXIN ON ACETYLCHOLINESTERASE DURING IN-VITRO NEUROMUSCULAR SYNAPTOGENESIS", Journal of the neurological sciences, 117(1-2), 1993, pp. 92-102

Abstract

Myasthenia gravis (MG) is mediated by circulating antibodies directedagainst acetylcholine receptor (AChR) but the antibody titre is poorly correlated with the clinical severity of the disease. We analysed acetylcholinesterase (AChE) activity, molecular forms and distribution during in vitro synaptogenesis, in the presence of sera from MG patient. We observed that the formation of AChE patches is inhibited in proportion to the anti-AChR antibody titre, whatever the clinical severity of the disease. The total activity and the proportion of the differentmolecular forms were unchanged suggesting that AChE level and distribution are controlled by independent mechanisms. To clarify the relationship between the mechanisms of AChE concentration during synaptogenesis and AChR concentration, we compared the effect of MG sera (receptors are internalised and degraded) and of the acetylcholine antagonist alpha-bungarotoxin (non-functional receptors are still present in the muscular membrane). In the presence of alpha-bungarotoxin, the number of AChR clusters, and AChE activity and concentration were equivalent to control values. The comparison of the results obtained with antibodies and alpha-bungarotoxin suggests that the presence and/or concentration of AChR is a necessary condition for normal concentration of AChE during synaptogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 15:42:08