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Titolo:
REGULATORY ASPECTS OF ESTERASE-6 ACTIVITY VARIATION IN SIBLING DROSOPHILA SPECIES
Autore:
KAROTAM J; OAKESHOTT JG;
Indirizzi:
QUEENS UNIV,DEPT BIOL KINGSTON K7L 5N6 ON CANADA CSIRO,DIV ENTOMOL CANBERRA ACT 2601 AUSTRALIA AUSTRALIAN NATL UNIV,DIV BIOCHEM & MOLEC BIOL CANBERRA ACT 2601 AUSTRALIA
Titolo Testata:
Heredity
, volume: 71, anno: 1993,
parte:, 1
pagine: 41 - 50
SICI:
0018-067X(1993)71:<41:RAOEAV>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; NATURAL-POPULATION; MOLECULAR ANALYSIS; JUVENILE-HORMONE; UNLINKED GENES; MELANOGASTER; ECDYSONE; LOCUS; EVOLUTION; SIMULANS;
Keywords:
ACTIVITY VARIATION; DROSOPHILA; ESTERASE-6; GENE REGULATION; GERM LINE TRANSFORMATION; INTERSPECIFIC COMPARISONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
J. Karotam e J.G. Oakeshott, "REGULATORY ASPECTS OF ESTERASE-6 ACTIVITY VARIATION IN SIBLING DROSOPHILA SPECIES", Heredity, 71, 1993, pp. 41-50

Abstract

Esterase 6 in Drosophila melanogaster, Drosophila simulans and Drosophila mauritiana is produced in several life stages and diverse tissues, but the major pulse of expression is in the sperm ejaculatory duct of adult males. Comparison of EST6 activity levels among several lines of D. melanogaster, D. simulans and D. mauritiana reveals two major quantitative differences among the species. First, newly eclosed femalesof both D. simulans and D. mauritiana show significantly higher EST6 activity than those of D. melanogaster. Secondly, 5-day-old adult D. simulans have significantly higher activities than D. mauritiana in both sexes and significantly higher activity than D. melanogaster in males. The genetic bases of the differences between D. melanogaster and the other species are investigated through germ line transfer of the D. simulans and D. mauritiana Est-6 genes plus 1.2 kb of 5' and 0.2 kb oftheir 3' flanking sequences into D. melanogaster. The newly eclosed female activities of the transformants resemble those of the two donor species, suggesting that the interspecific differences in this aspect of expression are due to cis-inherited factors contained within the transferred DNA. In contrast, the 5-day adult activity of the D. simulans transgene resembles the recipient species, D. melanogaster, suggesting that the difference between D. simulans and D. melanogaster in thisaspect of expression is due to trans-acting factors. We also find that third instar larval activities of the D. simulans transgene and 5-day male activities of the D. mauritiana transgene are lower than those of either parental species, suggesting that not all the promoter elements relevant to these aspects of expression are included in the transferred DNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 23:51:18