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Titolo:
ENDOTHELIAL TRANSDIFFERENTIATED PHENOTYPE AND CELL-CYCLE KINETICS OF AIDS-ASSOCIATED KAPOSI-SARCOMA CELLS
Autore:
WAY DL; WITTE MH; FIALA M; RAMIREZ G; NAGLE RB; BERNAS MJ; DICTOR M; BORGS P; WITTE CL;
Indirizzi:
UNIV ARIZONA,COLL MED,DEPT SURG,1501 N CAMPBELL AVE TUCSON AZ 85724 UNIV ARIZONA,COLL MED,DEPT PATHOL TUCSON AZ 85724 UNIV CALIF LOS ANGELES,DEPT NEUROL LOS ANGELES CA 90024 LUND UNIV,DEPT PATHOL S-22101 LUND SWEDEN
Titolo Testata:
Lymphology
fascicolo: 2, volume: 26, anno: 1993,
pagine: 79 - 89
SICI:
0024-7766(1993)26:2<79:ETPACK>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM CULTURE; LYMPHATIC ENDOTHELIUM; GROWTH-FACTOR; DNA; INVITRO; ORIGIN; TRANSFECTION; MARKERS; LESIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
D.L. Way et al., "ENDOTHELIAL TRANSDIFFERENTIATED PHENOTYPE AND CELL-CYCLE KINETICS OF AIDS-ASSOCIATED KAPOSI-SARCOMA CELLS", Lymphology, 26(2), 1993, pp. 79-89

Abstract

The nature of Kaposi sarcoma (KS) (vascular malignancy vs. discordantangiogenesis) and lineage of the progenitor cell remain unclear. Therefore, AIDS-KS enzyme isolate cultures were prepared from excised skinlesions. Endothelial marker positivity for Factor VIII related antigen (F8PAg), Ulex europaeus agglutinin (UEA), and angiotensin-convertingenzyme (ACE) were determined by fluorescence microscopy (FM) and flowcytometry (FCM). DNA cell-cycle analysis was performed using FCM. KS lesions showed large thick-walled channels (F8RAg and UEA strongly +),narrow vascular slits and thin-walled lakes (F8RAg and UEA weakly +),and non-prominent spindle cells (F8RAg and UEA almost uniformly negative). KS cultures yielded heterogenous populations of spindle, stellate, and flattened endothelial-like cells, displaying positivity for F8PAg (64 +/- 3%; mean +/- SE), UEA (40 +/- 9%), and ACE (81 +/- 9%). When injected subcutaneously in the nude mouse these cells failed to produce tumors. During contact inhibition induced quiescence, KS cultures exhibited a high G2M (18 +/- 3%) compared to non-KS (7 +/- 4%; p < 0.04), evidence of an altered proliferative potential consistent with a transdifferentiated or transformed phenotype.

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Documento generato il 01/12/20 alle ore 19:55:04