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Titolo:
INTENSITY AND ANISOTROPY DECAYS OF [LEU(5)] ENKEPHALIN TYROSYL FLUORESCENCE BY 10 GHZ FREQUENCY-DOMAIN FLUOROMETRY
Autore:
LAKOWICZ JR; GRYCZYNSKI I; LACZKO G; WICZK W;
Indirizzi:
UNIV MARYLAND,SCH MED,CTR FLUORESCENCE SPECT,DEPT BIOL CHEM,108 N GREENE ST BALTIMORE MD 21201
Titolo Testata:
Biophysical chemistry
fascicolo: 1, volume: 47, anno: 1993,
pagine: 33 - 40
SICI:
0301-4622(1993)47:1<33:IAADO[>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
TIME-RESOLVED FLUORESCENCE; PHASE-MODULATION FLUOROMETRY; PICOSECOND RESOLUTION; TRYPTOPHAN RESIDUES; PROTEIN DYNAMICS; DEPOLARIZATION; KINETICS; PEPTIDES; MOTIONS; H-1-NMR;
Keywords:
FREQUENCY-DOMAIN FLUOROMETRY; ANISOTROPY DECAYS; MOLECULAR DYNAMICS; FLUORESCENCE SPECTROSCOPY; [LEU(5)] ENKEPHALIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
J.R. Lakowicz et al., "INTENSITY AND ANISOTROPY DECAYS OF [LEU(5)] ENKEPHALIN TYROSYL FLUORESCENCE BY 10 GHZ FREQUENCY-DOMAIN FLUOROMETRY", Biophysical chemistry, 47(1), 1993, pp. 33-40

Abstract

The technique of 10 GHz frequency-domain fluorometry was used to resolve the complex picosecond intensity and anisotropy decays of the tyrosyl emission of [Leu5] enkephalin. Enhanced resolution of anisotropy decay was obtained by using acrylamide quenching of the tyrosyl fluorescence and global analysis of the frequency-domain anisotropy data obtained with different amounts of acrylamide. The data indicates a 44 ps correlation time for local tyrosine motions, and a 219 ps correlation time for overall rotational diffusion of the pentapeptide. Our data are consistent with an initial loss of fluorescence anisotropy from r0 =0.4 to a value of r0 = 0.326 occurring during the first two picoseconds after excitation.

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Documento generato il 04/12/20 alle ore 16:13:24