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Titolo:
COMPATIBLE SOLUTES OF HALOPHILIC EUBACTERIA - MOLECULAR PRINCIPLES, WATER-SOLUTE INTERACTION, STRESS PROTECTION
Autore:
GALINSKI EA;
Indirizzi:
UNIV BONN,INST MIKROBIOL & BIOTECHNOL,MECKENHEIMER 168 W-5300 BONN 1 GERMANY
Titolo Testata:
Experientia
fascicolo: 6-7, volume: 49, anno: 1993,
pagine: 487 - 496
SICI:
0014-4754(1993)49:6-7<487:CSOHE->2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTREME SALT CONCENTRATIONS; ORGANIC SOLUTES; GLYCINE BETAINE; ECTOTHIORHODOSPIRA-HALOCHLORIS; GLUTAMINE-SYNTHETASE; ENHANCED PROTECTION; OSMOTIC ADJUSTMENT; DIVALENT-CATIONS; PROTEINS; TREHALOSE;
Keywords:
HALOPHILIC HALOTOLERANT BACTERIA; COMPATIBLE SOLUTE; OSMOLYTE; OSMOADAPTATION; ENZYME STABILIZATION; STRESS PROTECTION; WATER STRUCTURE FORMER; KOSMOTROPIC SOLUTE;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
80
Recensione:
Indirizzi per estratti:
Citazione:
E.A. Galinski, "COMPATIBLE SOLUTES OF HALOPHILIC EUBACTERIA - MOLECULAR PRINCIPLES, WATER-SOLUTE INTERACTION, STRESS PROTECTION", Experientia, 49(6-7), 1993, pp. 487-496

Abstract

Compatible solutes are best described as organic osmolytes responsible for osmotic balance and at the same time compatible with the cells' metabolism. A comprehensive survey (using HPLC and NMR methods) on halophilic/halotolerant eubacteria has revealed the full diversity of compatible solutes employed in nature. Molecular principles derived from the spectrum of compounds found in the bacterial world may be summarized as follows. Compatible solutes are polar, highly soluble molecules and uncharged at physiological pH. With the exception of proline (a proteinogenic amino acid) they are characterized as amino acid derivatives of the following types: betaines, ectoines, N-acetylated diamino acids and N-derivatized carboxamides of glutamine. Using near-infrared spectroscopy we have also been able to demonstrate that compatible solutes are strong water-structure formers and as such probably excluded from the hydration shell of proteins. This ''preferential exclusion'' probably explains their function as effective stabilizers of the hydration shell of native proteins (protection against heating freezing and drying). Hence these typical products of halophilic eubacteria have a considerable potential as stabilizing/protecting agents on both molecular and whole-cell level. Thorough understanding of common structural principles and fundamental water-solute interactions will ultimately enable us to design novel highly efficient stress protectants and stabilizers of biomolecules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/10/20 alle ore 18:40:23