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Titolo:
TIF4631 AND TIF4632 - 2 YEAST GENES ENCODING THE HIGH-MOLECULAR-WEIGHT SUBUNITS OF THE CAP-BINDING PROTEIN COMPLEX (EUKARYOTIC INITIATION FACTOR-4F) CONTAIN AN RNA RECOGNITION MOTIF-LIKE SEQUENCE AND CARRY OUTAN ESSENTIAL FUNCTION
Autore:
GOYER C; ALTMANN M; LEE HS; BLANC A; DESHMUKH M; WOOLFORD JL; TRACHSEL H; SONENBERG N;
Indirizzi:
MCGILL UNIV,DEPT BIOCHEM MONTREAL H3G 1Y6 QUEBEC CANADA MCGILL UNIV,DEPT BIOCHEM MONTREAL H3G 1Y6 QUEBEC CANADA MCGILL UNIV,MCGILL CANC CTR MONTREAL H3G 1Y6 QUEBEC CANADA UNIV BERN,INST BIOCHEM & MOLEC BIOL CH-3000 BERN SWITZERLAND CARNEGIE MELLON UNIV,DEPT BIOL SCI PITTSBURGH PA 15213
Titolo Testata:
Molecular and cellular biology
fascicolo: 8, volume: 13, anno: 1993,
pagine: 4860 - 4874
SICI:
0270-7306(1993)13:8<4860:TAT-2Y>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFECTED HELA-CELLS; MESSENGER-RNA; SACCHAROMYCES-CEREVISIAE; TRANSLATION INITIATION; WHEAT-GERM; RIBOSOMAL-PROTEINS; SPLICING INVITRO; NUCLEAR EXTRACT; ISOZYME FORM; POLIOVIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
96
Recensione:
Indirizzi per estratti:
Citazione:
C. Goyer et al., "TIF4631 AND TIF4632 - 2 YEAST GENES ENCODING THE HIGH-MOLECULAR-WEIGHT SUBUNITS OF THE CAP-BINDING PROTEIN COMPLEX (EUKARYOTIC INITIATION FACTOR-4F) CONTAIN AN RNA RECOGNITION MOTIF-LIKE SEQUENCE AND CARRY OUTAN ESSENTIAL FUNCTION", Molecular and cellular biology, 13(8), 1993, pp. 4860-4874

Abstract

The 5' ends of eukaryotic mRNAs are blocked by a cap structure, m7GpppX (where X is any nucleotide). The interaction of the cap structure with a cap-binding protein complex is required for efficient ribosome binding to the mRNA. In Saccharomyces cerevisiae, the cap-binding protein complex is a heterodimer composed of two subunits with molecular masses of 24 (eIF-4E, CDC33) and 150 (p150) kDa. p150 is presumed to be the yeast homolog of the p220 component of mammalian eIF-4F. In this report, we describe the isolation of yeast gene TIF4631, which encodes p150, and a closely related gene, TIF4632. TIF4631 and TIF4632 are 53%identical overall and 80% identical over a 320-amino-acid stretch in their carboxy-terminal halves. Bo proteins contain sequences resembling the RNA recognition motif and auxiliary domains that are characteristic of a large family of RNA-binding proteins. tif4631-disrupted strains exhibited a slow-growth, cold-sensitive phenotype, while disruptionof TIF4632 failed to show any phenotype under the conditions assayed. Double gene disruption engendered lethality, suggesting that the two genes are functionally homologous and demonstrating that at least one of them is essential for viability. These data are consistent with a critical role for the high-molecular-weight subunit of putative yeast eIF-4F in translation. Sequence comparison of TIF4631, TIF4632, and thehuman eIF-4F p220 subunit revealed significant stretches of homology. We have thus cloned two yeast homologs of mammalian p220.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:57:49