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Titolo:
CELLULAR-LOCALIZATION OF MESSENGER-RNAS IN RAT TESTIS - APPLICATION OF DIGOXIGENIN-LABELED RIBONUCLEOTIDE PROBES TO EMBEDDED TISSUE
Autore:
MILLAR MR; SHARPE RM; MAGUIRE SM; SAUNDERS PTK;
Indirizzi:
MRC,CTR REPROD BIOL,REPROD BIOL UNIT,37 CHALMERS ST EDINBURGH EH3 9EWMIDLOTHIAN SCOTLAND
Titolo Testata:
Cell and tissue research
fascicolo: 2, volume: 273, anno: 1993,
pagine: 269 - 277
SICI:
0302-766X(1993)273:2<269:COMIRT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSITU HYBRIDIZATION; PROTEIN-2;
Keywords:
SPERMATOGENESIS; CYCLIC PROTEIN 2; TRANSITION PROTEIN-2; CYTOCHROME-C OXIDASE; SERTOLI CELL; SPERMATIDS; PACHYTENE SPERMATOCYTES; RAT (WISTAR);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
M.R. Millar et al., "CELLULAR-LOCALIZATION OF MESSENGER-RNAS IN RAT TESTIS - APPLICATION OF DIGOXIGENIN-LABELED RIBONUCLEOTIDE PROBES TO EMBEDDED TISSUE", Cell and tissue research, 273(2), 1993, pp. 269-277

Abstract

In an attempt to identify key changes involved in normal spermatogenesis we have developed methods to enable the study of gene expression by the various subpopulations of testicular cells by use of in-situ hybridisation histochemistry. The use of digoxigenin-labelled ribonucleotide and oligonucleotide probes on testicular tissue perfusion-fixed with Bouin's fixative and embedded in paraffin, polystyrene or methacrylate, has been used to accurately localise three transcripts to three different cell types (Sertoli cells, pachytene spermatocytes, and step 7-12 spermatids) within the seminiferous tubule. The ability to produce semi-thin sections of polystyrene- or methacrylate-embedded tissue and successfully to apply digoxigenin-labelled ribonucleotide or oligonucleotide probes resulted in far greater resolution and unequivocal localisation of mRNA in testicular cells than was previously possible byuse of thicker paraffin or frozen sections hybridised with S-35-labelled riboprobes. A comparison of the different embedding media versus digoxigenin-labelled oligonucleotide or ribonucleotide probes is made and we demonstrate the relative sensitivities and merits of each combination.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 17:25:14