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Titolo:
USE OF PRIMARY CULTURES OF SALMON HEPATOCYTES FOR THE STUDY OF HORMONAL-REGULATION OF INSULIN-LIKE GROWTH FACTOR-I EXPRESSION IN-VITRO
Autore:
DUAN CM; HANZAWA N; TAKEUCHI Y; HAMADA E; MIYACHI S; HIRANO T;
Indirizzi:
UNIV WASHINGTON,SCH FISHERIES HF-15 SEATTLE WA 98195 MARINE BIOTECHNOL INST,KAMAISHI LABS KAMAISHI IWATE 026 JAPAN UNIV TOKYO,OCEAN RES INST TOKYO 164 JAPAN
Titolo Testata:
Zoological science
fascicolo: 3, volume: 10, anno: 1993,
pagine: 473 - 480
SICI:
0289-0003(1993)10:3<473:UOPCOS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED LIVER-CELLS; COHO SALMON; NUCLEOTIDE-SEQUENCE; BRANCHIAL CARTILAGE; ANGUILLA-JAPONICA; SULFATE UPTAKE; JAPANESE EEL; STIMULATION; ACID; SERUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
C.M. Duan et al., "USE OF PRIMARY CULTURES OF SALMON HEPATOCYTES FOR THE STUDY OF HORMONAL-REGULATION OF INSULIN-LIKE GROWTH FACTOR-I EXPRESSION IN-VITRO", Zoological science, 10(3), 1993, pp. 473-480

Abstract

Hepatocytes isolated from sockeye salmon (Oncorhynchus nerka) were seeded on an uncoated plastic dish with a positively charged surface, and cultured in hormone-free defined medium. Initially single cells began to aggregate and became flat after being attached to the dish. Afterapproximately 6 days in culture, hepatocytes formed a monolayer sheetwith cells in side-by-side contact, and remained in this form for at least 2 weeks. Hepatocytes in multicellular spheroids were also observed. The viability of the cells remained high (85-90%) during culture as judged by trypan blue exclusion. The cells actively incorporated [H-3]leucine into cellular and secreted proteins. Salmon and bovine insulins stimulated the leucine incorporation in a dose-dependent manner, suggesting that the cells remained responsive to hormones during culture. The basal IGF-I mRNA level in cultured hepatocytes was much lower than that of in vivo status. Salmon GH increased the level of IGF-I mRNA, whereas salmon prolactin had no effect. These findings suggest thatthe primary culture system of salmon hepatocytes can be used for the in vitro study of hormonal regulation of IGF-I gene expression in salmon.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:56:06