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Titolo: DETECTION OF THE C-PROTEIN GENE AMONG GROUP B-STREPTOCOCCI USING PCR
Autore: MAWN JA; SIMPSON AJ; HEARD SR;
- Indirizzi:
- UNIV GREENWICH,SCH BIOL & CHEM SCI,WELLINGTON ST LONDON SE18 6PF ENGLAND ST BARTHOLOMEWS HOSP,DEPT MED MICROBIOL LONDON EC1A 7BE ENGLAND
- Titolo Testata:
- Journal of Clinical Pathology
fascicolo: 7,
volume: 46,
anno: 1993,
pagine: 633 - 636
- SICI:
- 0021-9746(1993)46:7<633:DOTCGA>2.0.ZU;2-J
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- MOUSE-PROTECTIVE ANTIBODIES; POLYMERASE CHAIN-REACTION; ANTIGENS; DNA; AMPLIFICATION; INFECTION; IGA;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Citazioni:
- 24
- Recensione:
- Indirizzi per estratti:
-
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- Citazione:
- J.A. Mawn et al., "DETECTION OF THE C-PROTEIN GENE AMONG GROUP B-STREPTOCOCCI USING PCR", Journal of Clinical Pathology, 46(7), 1993, pp. 633-636
Abstract
Aim-To develop a polymerase chain reaction (PCR) for the specific detection of the C protein gene in strains of group B Streptococcus. Methods-A single primer pair derived from the nucleotide sequence of the IgA binding beta antigen of the C protein complex permitted the specific amplification of a 592 base pair DNA fragment from the C protein gene. After 35 cycles of amplification this product could be detected by agarose gel electrophoresis. Southern blot hybridisation confirmed that this product was the C protein gene. Results-PCR detected the C protein gene in 75 (63%) of 119 strains of group B streptococci analysed. The product was not detected in other Gram positive organisms, showingthat this PCR assay was highly specific. The sensitivity of the assaywas satisfactory to a dilution of 1 in 10 000 of extracted DNA. Conclusions-The C protein of group B streptococci is associated with neonatal sepsis. The specific detection of the C protein gene by PCR may help identify which strains are likely to be associated with infection bythe organism.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 18:48:23