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Titolo:
A UTERINE MUSCLE CONTRACTILE SUBSTANCE OBTAINED FROM A HUMAN PLACENTAL CDNA LIBRARY
Autore:
SAKAI A; MAKINO T; KATO Y; KATO T; MOTOYAMA S; SAITO S; IIZUKA R; NOZAWA S;
Indirizzi:
NICHHD,ENDOCRINOL & REPROD RES BRANCH BETHESDA MD 20892
Titolo Testata:
Gynecological endocrinology
fascicolo: 2, volume: 7, anno: 1993,
pagine: 77 - 82
SICI:
0951-3590(1993)7:2<77:AUMCSO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Keywords:
PLACENTA; CDNA; UTERINE CONTRACTION; OXYTOCIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
A. Sakai et al., "A UTERINE MUSCLE CONTRACTILE SUBSTANCE OBTAINED FROM A HUMAN PLACENTAL CDNA LIBRARY", Gynecological endocrinology, 7(2), 1993, pp. 77-82

Abstract

A cDNA clone coding for an oxytocin-like substance was prepared from a human placental cDNA library. using oxytocin antiserum. The cDNA size was approximately 900 bp. A mammalian expression vector containing the cDNA was constructed and transfected into Chinese hamster ovary (CHO) cells. The expression of immunoreactivity to oxytocin antiserum wasobserved by radioimmunoassay. The cultured medium of the transfected cells was assayed for uterine muscle contractile bioactivity using a Magnus apparatus. Bioactivity was eliminated by incubation with oxytocin antiserum. Thus, the cDNA done, screened with oxytocin antibody, is surmised to code for an oxytocin-like activity, but the nucleotide andamino acid sequences responsible for the biological activity remain to be clarified.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 21:52:48