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Titolo:
STUDIES OF TISSUE-CULTURED SLICED DERMIS AS A SKIN SUBSTITUTE
Autore:
ONO I; GUNJI H; MARUYAMA K; SUZUKI M; KANEKO F;
Indirizzi:
FUKUSHIMA MED COLL,DEPT DERMATOL,DIV PLAST & RECONSTRUCT SURG,HIKARIGAOKO 1 FUKUSHIMA 96012 JAPAN
Titolo Testata:
Burns
fascicolo: 4, volume: 19, anno: 1993,
pagine: 283 - 288
SICI:
0305-4179(1993)19:4<283:SOTSDA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
I. Ono et al., "STUDIES OF TISSUE-CULTURED SLICED DERMIS AS A SKIN SUBSTITUTE", Burns, 19(4), 1993, pp. 283-288

Abstract

A dermis slicer designed by the authors enabled us to prepare about 10 sheets of sliced dermal grafts (SDG), 300 mum thick, from the dermisharvested from the back or buttocks of adult patients during operations. Such a sliced dermal sheet was stretched with one surface stuck onthe base of a culture dish. It was then incubated in Dulbecco's essential medium for tissue culture, to which epidermal growth factor had been added. By the first week only its upper side was epithelialized from epithelial components in sliced dermis. The formation of basement membrane with anchoring fibrils was confirmed by electron microscopy. The appearance of type IV collagen and laminin was observed between epithelialized basal cells and the dermal layer. Thus, it is thought thatthe SDG is useful not only for immediate grafting, when epithelialization follows, but also as a substitute for free split thickness skin grafts following tissue culture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:31:41