Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PRINCIPAL XENOBIOTIC-METABOLIZING ENZYME-SYSTEMS IN HUMAN HEAD AND NECK SQUAMOUS-CELL CARCINOMA
Autore:
JANOT F; MASSAAD L; RIBRAG V; DEWAZIERS I; BEAUNE PH; LUBOINSKI B; PARISE O; GOUYETTE A; CHABOT GG;
Indirizzi:
INST GUSTAVE ROUSSY,DEPT HEAD & NECK SURG,CNRS,URA 147,INSERM,U140 F-94805 VILLEJUIF FRANCE INST GUSTAVE ROUSSY,DEPT HEAD & NECK SURG,CNRS,URA 147,INSERM,U140 F-94805 VILLEJUIF FRANCE INST GUSTAVE ROUSSY,CLIN PHARMACOL LAB,CNRS,URA 147,INSERM,U140 F-94805 VILLEJUIF FRANCE NECKER ENFANTS MALAD UNIV HOSP,INSERM,U75,PHARMACOL & METAB BIOCHEM LAB F-75730 PARIS 15 FRANCE
Titolo Testata:
Carcinogenesis
fascicolo: 7, volume: 14, anno: 1993,
pagine: 1279 - 1283
SICI:
0143-3334(1993)14:7<1279:PXEIHH>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTATHIONE-S-TRANSFERASE; HUMAN-LIVER; UTERINE CERVIX; GST-PI; CANCER; TUMOR; EXPRESSION; TISSUE; MARKER; CYTOCHROME-P-450;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
F. Janot et al., "PRINCIPAL XENOBIOTIC-METABOLIZING ENZYME-SYSTEMS IN HUMAN HEAD AND NECK SQUAMOUS-CELL CARCINOMA", Carcinogenesis, 14(7), 1993, pp. 1279-1283

Abstract

To better understand drug and carcinogen metabolism pathways in head and neck squamous cell carcinoma we assayed the principal drug- and carcinogen-metabolizing enzyme systems in both tumors and their corresponding adjacent non-tumoral tissues. Cytochromes P450 (1A1/A2, 2B1/B2, 2C8-10, 2E1, 3A4), epoxide hydrolase and glutathione S-transferases (GST-alpha, GST-mu, GST-pi) were assayed by immunoblotting. GST activity, total glutathione, UDP-glucuronosyltransferase, beta-glucuronidase, sulfotransferase and sulfatase, were determined by spectral assays. Results showed the absence of all probed cytochromes P450 in tumors and non-tumoral tissues, including P450 1A1/1A2 known to be involved in tobacco-related carcinogenesis. No statistical difference was noted between tumors and adjacent non-tumoral tissues for most enzymes studied (GST-alpha, GST-mu, GST-pi, GST activity, UDP-glucuronosyltransferase, beta-glucuronidase, sulfotransferase and sulfatase). However, total glutathione concentrations were significantly higher (P < 0.05) in tumors (47 +/- 20 nmol/mg protein) than in nontumoral tissues (19 +/- 9). On the contrary, epoxide hydrolase was significantly less expressed in tumors (18 +/- 9 mug/mg protein) compared to corresponding non-tumoraltissues (37 +/- 9). These data provide new information concerning human head and neck cancer biology that could possibly have clinical implications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 08:35:40