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Titolo:
AN IMMUNODOMINANT CD4-CELL SITE ON THE NUCLEOCAPSID PROTEIN OF MURINECORONAVIRUS CONTRIBUTES TO PROTECTION AGAINST ENCEPHALOMYELITIS( T)
Autore:
WEGE H; SCHLIEPHAKE A; KORNER H; FLORY E; WEGE H;
Indirizzi:
UNIV WURZBURG,INST VIROL & IMMUNOBIOL,VERSBACHER STR 7 W-7078 WURZBURG GERMANY
Titolo Testata:
Journal of General Virology
, volume: 74, anno: 1993,
parte:, 7
pagine: 1287 - 1294
SICI:
0022-1317(1993)74:<1287:AICSOT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; MOUSE HEPATITIS-VIRUS; INDUCED DEMYELINATING ENCEPHALOMYELITIS; ESCHERICHIA-COLI; LETHAL INFECTION; LYMPHOCYTES-T; JHM; RATS; ENCEPHALITIS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
H. Wege et al., "AN IMMUNODOMINANT CD4-CELL SITE ON THE NUCLEOCAPSID PROTEIN OF MURINECORONAVIRUS CONTRIBUTES TO PROTECTION AGAINST ENCEPHALOMYELITIS( T)", Journal of General Virology, 74, 1993, pp. 1287-1294

Abstract

The murine coronavirus neurotropic strain JHM (MHV-JHM) nucleocapsid (N) protein induces a strong T-helper cell response in Lewis rats. It has been shown previously that N-specific CD4+ T cells can confer protection against acute disease upon transfer to otherwise lethally infected rats. To define the major antigenic regions that elicit this T cell response, truncated fragments of N protein were expressed from a bacterial expression vector and employed as T cell antigens. Lymphocytes from either MHV-JHM-infected or immunized rats were stimulated in culture with virus antigen, grown and tested for their specificity to the N protein fragments. The carboxy-terminally located C4-N fragment (95 amino acids) induced the most pronounced proliferative response irrespective of whether the lymphocyte culture was derived from immunized orMHV-JHM-infected rats. We established T cell lines specific for the truncated N protein fragments and tested their potential to mediate protection by transfer experiments. Only the T cell line C4-N and the T cell line specific for the full-length N protein were protective. By contrast, all truncated N protein fragments elicited a humoral immune response and contained antigenic sites recognized by antibodies from diseased rats.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:55:39