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Titolo:
INDUCTION OF CYTOLYTIC T-LYMPHOCYTES DIRECTED TOWARDS THE V3 LOOP OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 EXTERNAL GLYCOPROTEIN-GP120 BYP55(GAG) V3 CHIMERIC VACCINIA VIRUSES/
Autore:
WAGNER R; BOLTZ T; DEML L; MODROW S; WOLF H;
Indirizzi:
UNIV REGENSBURG,INST MED MICROBIOL W-8400 REGENSBURG GERMANY UNIV ULM,DEPT VIROL W-7900 ULM GERMANY
Titolo Testata:
Journal of General Virology
, volume: 74, anno: 1993,
parte:, 7
pagine: 1261 - 1269
SICI:
0022-1317(1993)74:<1261:IOCTDT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIV-1 INFECTION INVITRO; MONOCLONAL-ANTIBODIES; ENVELOPE PROTEIN; SYNTHETIC PEPTIDES; SEROPOSITIVE INDIVIDUALS; INFLUENZA HEMAGGLUTININ; CELL RECOGNITION; ACID SEQUENCE; CHIMPANZEES; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
R. Wagner et al., "INDUCTION OF CYTOLYTIC T-LYMPHOCYTES DIRECTED TOWARDS THE V3 LOOP OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 EXTERNAL GLYCOPROTEIN-GP120 BYP55(GAG) V3 CHIMERIC VACCINIA VIRUSES/", Journal of General Virology, 74, 1993, pp. 1261-1269

Abstract

T cell-mediated cytotoxicity may play an important role in controlling infection by human immunodeficiency virus (HIV). In order to study the ability of rationally designed antigens to induce cytolytic T lymphocytes (CTLs) we replaced stretches of 30 to 50 amino acids at the p17-MA/p24-CA cleavage site, within the p24-CA moiety and within the p6-LI portion of the HIV type 1 p55gag precursor by the third variable domain (V3) of the external glycoprotein gp120. This site is known to be a target for CTL attack in mice and humans. The chimeric antigens wererecombined into highly attenuated vaccinia viruses in order to investigate class I major histocompatibility complex (MHC)-restricted presentation of antigenic V3 peptides. Immunoprecipitation and Western blot analysis of the group-specific antigen (p55gag)/V3 chimeric proteins demonstrated significant differences in the accessibility of the V3 domain for a monoclonal antibody or polyclonal V3-specific antisera, depending on the position of the V3 loop within the p55gag carrier protein. Immunization of BALB/c mice with three variants of p55gag/V3 recombinant vaccinia virus, however, resulted in a comparable priming of CD4-CD8+ CTLs in vivo irrelevant of the position of the V3 loop within p55gag. Local conformational changes, including the V3 domain within thep55gag/V3 chimeras, did not demonstrate a significant effect on V3-specific lysis of the target cells when compared to the authentic gp120 envelope protein. Class I MHC-restricted CTLs induced by a V3 consensus sequence cross-reacted perfectly with the LAI strain-derived V3 loopsequence. These data indicate that the combination of selected epitopes (V3) with immunologically relevant complex carrier proteins (p55gag) can be accomplished without the loss of biological activity.

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Documento generato il 01/10/20 alle ore 00:23:12