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Titolo:
DIAGNOSTIC-VALUE OF AMPLIFICATION OF HUMAN CYTOMEGALOVIRUS DNA FROM GASTROINTESTINAL BIOPSIES FROM HUMAN IMMUNODEFICIENCY VIRUS-INFECTED PATIENTS
Autore:
COTTE L; DROUET E; BISSUEL F; DENOYEL GA; TREPO C;
Indirizzi:
HOP HOTEL DIEU,HEPATOGASTROENTEROL & AIDS CLIN & RES UNIT LYON FRANCE INST PASTEUR,INSERM,U271 LYON FRANCE INST PASTEUR,VIROL UNIT LYON FRANCE
Titolo Testata:
Journal of clinical microbiology
fascicolo: 8, volume: 31, anno: 1993,
pagine: 2066 - 2069
SICI:
0095-1137(1993)31:8<2066:DOAOHC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSITU HYBRIDIZATION; AIDS; COLITIS; IMMUNOHISTOCHEMISTRY; CULTURE; DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
L. Cotte et al., "DIAGNOSTIC-VALUE OF AMPLIFICATION OF HUMAN CYTOMEGALOVIRUS DNA FROM GASTROINTESTINAL BIOPSIES FROM HUMAN IMMUNODEFICIENCY VIRUS-INFECTED PATIENTS", Journal of clinical microbiology, 31(8), 1993, pp. 2066-2069

Abstract

In order to assess the value of human cytomegalovirus (HCMV) DNA amplification of gastrointestinal biopsies, we studied 57 human immunodeficiency virus-infected patients with and without gastrointestinal HCMV diseases. After DNA extraction, a 406-bp fragment from the unique short region of the HCMV genome was amplified by 35 cycles of polymerase chain reaction (PCR) and semiquantified from 80 to 80,000 HCMV genomic copies. Among 12 non-AIDS patients, the PCR assay was negative for 11 of 12 duodenal and 8 of 8 colorectal samples. It was also negative for28 of 31 duodenal and 12 of 15 colorectal samples from 31 AIDS patients without gastrointestinal HCMV diseases. Among 14 AIDS patients withgastrointestinal HCMV diseases, the PCR assay was positive for 12 of 12 patients with HCMV duodenitis and for 13 of 13 patients with HCMV colitis. Results were dichotomized between high and low HCMV-DNA copy numbers. For duodenitis, sensitivity was 92% and specificity was 100%. For colitis, sensitivity was 92% and specificity was 93%. Specificity and sensitivity were not influenced by shedding status for HCMV or by other gastrointestinal infections. HCMV DNA amplification of gastrointestinal biopsies is a sensitive and specific tool for the diagnosis ofgastrointestinal HCMV diseases in AIDS patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 14:14:17