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Titolo:
ISOLATION AND CHARACTERIZATION OF A NOVEL CDNA WHICH IDENTIFIES BOTH NEURAL-SPECIFIC AND UBIQUITOUSLY EXPRESSED GS-ALPHA MESSENGER-RNAS
Autore:
HABECKER BA; MARTIN JM; NATHANSON NM;
Indirizzi:
UNIV WASHINGTON,SCH MED,DEPT PHARMACOL,SJ-30 SEATTLE WA 98195 UNIV WASHINGTON,SCH MED,DEPT PHARMACOL,SJ-30 SEATTLE WA 98195
Titolo Testata:
Journal of neurochemistry
fascicolo: 2, volume: 61, anno: 1993,
pagine: 712 - 717
SICI:
0022-3042(1993)61:2<712:IACOAN>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL TRANSDUCTION PROTEIN; MOLECULAR-CLONING; DNA; SEQUENCE; SUBUNITS; FAMILY;
Keywords:
G-PROTEIN; DIFFERENTIAL SPLICING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
B.A. Habecker et al., "ISOLATION AND CHARACTERIZATION OF A NOVEL CDNA WHICH IDENTIFIES BOTH NEURAL-SPECIFIC AND UBIQUITOUSLY EXPRESSED GS-ALPHA MESSENGER-RNAS", Journal of neurochemistry, 61(2), 1993, pp. 712-717

Abstract

Heterotrimeric G proteins consisting of alpha, beta, and gamma subunits couple sensory, hormone, and neurotransmitter receptors to intracellular and transmembrane effectors. Several splicing variants of the G(S) (the G protein that stimulates adenylyl cyclase) a subunit (G(Salpha)) have been described. Some of these couple receptors to stimulationof adenylyl cyclase and Ca2+ channels, whereas others encode truncated proteins whose functions are not currently defined. We describe a 1321N1 human astrocytoma cDNA clone for a novel G(Salpha) isoform isolated from astrocytoma cells (G(astro)) that is identical to G(Salpha)-1 with the exception of a novel 5' sequence extending into the previously described exon 1 of G(Salpha), a single base change, and an alternative polyadenylation site. Analysis by northern blotting and reverse transcription/PCR confirms the presence of an mRNA corresponding to thiscDNA in astrocytoma cells. Additional northern analysis indicates that G(astro) recognizes two novel G(Salpha) mRNAs in the rat: a 2.0-kb mRNA expressed only in neural and neuroendocrine tissues and a 1.8-kb mRNA that is ubiquitously expressed. Functional analysis of G(astro) iscomplicated by the apparent insertion of alphoid satellite DNA into the transcription unit. The resulting cDNA encodes a truncated protein that may be translated from the methionine in exon 2 as previously described.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:08:36