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Titolo:
INCREASE IN NEURITE FORMATION AND ACETYLCHOLINE-RELEASE BY TRANSFECTION OF GROWTH-ASSOCIATED PROTEIN-43 CDNA INTO NG108-15 CELLS
Autore:
KUMAGAITOHDA C; TOHDA M; NOMURA Y;
Indirizzi:
HOKKAIDO UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
Journal of neurochemistry
fascicolo: 2, volume: 61, anno: 1993,
pagine: 526 - 532
SICI:
0022-3042(1993)61:2<526:IINFAA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINASE-C SUBSTRATE; GAP-43 GENE-EXPRESSION; NERVE GROWTH; PC12 CELLS; NORADRENALINE RELEASE; SUBCELLULAR FRACTION; MEMBRANE SKELETON; PLASMA-MEMBRANES; MESSENGER-RNA; PHORBOL ESTER;
Keywords:
GROWTH-ASSOCIATED PROTEIN-43; NEURITE FORMATION; ACETYLCHOLINE RELEASE; NG108-15 CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
C. Kumagaitohda et al., "INCREASE IN NEURITE FORMATION AND ACETYLCHOLINE-RELEASE BY TRANSFECTION OF GROWTH-ASSOCIATED PROTEIN-43 CDNA INTO NG108-15 CELLS", Journal of neurochemistry, 61(2), 1993, pp. 526-532

Abstract

We previously reported that growth-associated protein-43 (GAP-43) could be involved in the maintenance of elongated neurites and that a decline in protein kinase C activity may be involved in accumulation of GAP-43. In the present study, to clarify the functional significance ofGAP-43 for neurite maintenance and acetylcholine (ACh) release, we prepared NG-G11 cells by transfection of GAP-43 cDNA into NG108-15 cells. NG-G11 cells expressed GAP-43 mRNA at levels approximately twice that in nontransfected or vector-transfected cells under control conditions and after treatment with dibutyryl cyclic AMP (diBu-cAMP) or 12-O-tetradecanoylphorbol 13-acetate (TPA) plus diBu-cAMP. Neurite outgrowthafter addition of diBu-cAMP was greater in NG-G11 than in control cells. In NG-G11 cells, neurites elongated by treatment with diBu-cAMP for 72 h were maintained after removal of the drug. Treatment with TPA plus diBu-cAMP for 24 h induced neurite outgrowth in NG-G11 cells, although control cells required 72 h. Depolarization by 50 mM KCl induced ACh release in both NG-G11 and control cells treated with diBu-cAMP orTPA/diBu-cAMP. Although removal of the drugs following diBu-cAMP treatment reversed ACh release to nontreated levels in control cells, a high-K+-induced level of ACh release remained in NG-G11 cells after removal of diBu-cAMP. ACh release induced by TPA plus diBu-cAMP for 24 h was further enhanced after removal of the drugs in NG-G11 cells, but itwas not seen in control cells. These results suggest that levels of GAP-43 mRNA are correlated with neurite maintenance and the level of ACh release. Thus, GAP-43 may be involved in neuronal differentiation inNG108-15 cells.

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Documento generato il 25/10/20 alle ore 02:29:16