Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
HELPER T-CELL RECOGNITION OF HIV-1 TAT SYNTHETIC PEPTIDES
Autore:
BLAZEVIC V; RANKI A; MATTINEN S; VALLE SL; KOSKIMIES S; JUNG G; KROHN KJE;
Indirizzi:
UNIV TAMPERE,INST BIOMED SCI,POB 607 SF-33101 TAMPERE FINLAND UNIV HELSINKI,DEPT DERMATOL & VENEREAL DIS SF-00100 HELSINKI 10 FINLAND FINNISH RED CROSS & BLOOD TRANSFUS SERV HELSINKI FINLAND UNIV TUBINGEN,INST ORGAN CHEM W-7400 TUBINGEN 1 GERMANY
Titolo Testata:
Journal of acquired immune deficiency syndromes
fascicolo: 8, volume: 6, anno: 1993,
pagine: 881 - 890
SICI:
0894-9255(1993)6:8<881:HTROHT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; MHC CLASS-II; SECONDARY-STRUCTURE; ANTIGENIC SITES; POSITIVE SERA; HTLV-III; INFECTION; PROTEIN; EPITOPES; SEQUENCE;
Keywords:
T-CELL; T-CELL EPITOPE; TAT; HIV-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
V. Blazevic et al., "HELPER T-CELL RECOGNITION OF HIV-1 TAT SYNTHETIC PEPTIDES", Journal of acquired immune deficiency syndromes, 6(8), 1993, pp. 881-890

Abstract

The regulatory proteins coded by the human immunodeficiency virus, (HIV)-1 genome are expressed by the infected cells before the initiationof the synthesis of structural proteins and thus immune response directed against these proteins could destroy infected cells before the release of infectious virions. The evaluation of T-lymphocyte responses toward Tat, one of the main HIV-1 regulatory proteins, is therefore ofinterest. We selected a group of HIV-infected patients with retained response to the recall antigen purified protein derivative and tested their CD4+ helper T-cell response toward recombinant Tat and toward 12soluble synthetic partially overlapping 15-16-mer Tat peptides in a proliferation assay. Three peptides (amino acids 17-32, 33-48, and 65-80) were significantly recognized by the helper T-cells from infected individuals but not by the nine HIV-1-negative control persons. Nine ofthe 14 patients (64%) responded to at least one of these Tat peptides. Of the identified immunodominant peptides containing T-cell epitopes, one (aa 65-80) was recognized in association with human leukocyte antigens DR-2 allele, while the others appeared to be promiscuous and were equally recognized in association with several DR molecules. The identified immunogenic peptides were analyzed for the predicted presenceof T-cell antigenic sites by several algorithms and positive correlation was detected for each peptide. Our results thus indicate that Tat protein can induce a cell-mediated immune response and identify three peptides containing T-cell epitopes that may be of importance in vaccine development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:01:38