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Titolo:
OLIGOSACCHARIDE SPECIFICITY OF NORMAL HUMAN HEPATOCYTE ALPHA-1-3 FUCOSYL-TRANSFERASE
Autore:
JEZEQUELCUER M; NGUYENCONG H; BIOU D; DURAND G;
Indirizzi:
HOP BICHAT,BIOCHIM LAB A,46 RUE HUCHARD F-75877 PARIS 18 FRANCE UNIV PARIS 11,BIOCHIM LAB F-92290 CHATENAY MALABRY FRANCE
Titolo Testata:
Biochimica et biophysica acta
fascicolo: 3, volume: 1157, anno: 1993,
pagine: 252 - 258
SICI:
0006-3002(1993)1157:3<252:OSONHH>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAGNETIC-RESONANCE SPECTROSCOPY; ASIALO-CARBOHYDRATE UNITS; GAS-LIQUID CHROMATOGRAPHY; SIALYL-OLIGOSACCHARIDES; H-1-NMR SPECTROSCOPY; METHYL GLYCOSIDES; GOLGI-APPARATUS; SUGAR CHAINS; L-FUCOSIDASE; LIVER;
Keywords:
FUCOSYL-TRANSFERASE; HEPATOCYTE; HUMAN; LEWIS; OLIGOSACCHARIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
M. Jezequelcuer et al., "OLIGOSACCHARIDE SPECIFICITY OF NORMAL HUMAN HEPATOCYTE ALPHA-1-3 FUCOSYL-TRANSFERASE", Biochimica et biophysica acta, 1157(3), 1993, pp. 252-258

Abstract

A purified alpha1-3 fucosyltransferase (alpha1-3 FT) was recovered inthe Golgi fraction of isolated hepatocytes from normal human liver tissue. The efficiency of purification was controled by measurement of fucose transfer to asialotransferrin (for alpha1-3 FT), to phenyl-beta-D-galactose (for alpha1-2 FT) and to 2' fucosyl lactose (for alpha1-3/4 FT). The initial hepatocyte isolation step got rid of 97% and 94% ofalpha1-2 and alpha1-3/4 total liver FT, respectively. After Golgi enrichment (26-fold purification and a yield of 7.6%), alpha1-3 FT extract expressed a specific activity of 2 pM/min per mg protein. When incubated in optimized conditions with type 1, 2 or 6 oligosaccharide acceptors (10 mM), hepatocellular alpha-3 FT efficiently transferred fucoseto N-acetyllactosamine and its 3' sialylated derivative, but poorly to lactose. When incubated with neutral or sialylated biantennary N-glycans, the enzyme expressed the highest affinity (K(m) = 0.38 mM) for the 3' bisialylated derivative. This suggests that hepatocellular alpha1-3 FT is involved in the synthesis of sialosyl Le(x) determinants on cirrhotic alpha1AGP.

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Documento generato il 05/07/20 alle ore 09:29:57