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Titolo:
HIPPOCAMPAL REGULATION OF THE SURVIVAL AND MORPHOLOGICAL DEVELOPMENT OF LOCUS-CERULEUS NEURONS IN DISSOCIATED CELL-CULTURE
Autore:
ROBINSON LJ; BLACK IB; DREYFUS CF;
Indirizzi:
UMDNJ,ROBERT WOOD JOHNSON MED SCH,DEPT NEUROSCI & CELL BIOL,675 HOES LANE PISCATAWAY NJ 08854 UMDNJ,ROBERT WOOD JOHNSON MED SCH,DEPT NEUROSCI & CELL BIOL,675 HOES LANE PISCATAWAY NJ 08854 CORNELL UNIV,MED CTR,COLL MED,DEPT NEUROL,DIV DEV NEUROL NEW YORK NY 10021
Titolo Testata:
Journal of comparative neurology
fascicolo: 4, volume: 333, anno: 1993,
pagine: 567 - 577
SICI:
0021-9967(1993)333:4<567:HROTSA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; MESENCEPHALIC DOPAMINERGIC-NEURONS; IMMUNO-CYTOCHEMICAL LOCALIZATION; MOUSE SYMPATHETIC-GANGLION; FIMBRIA-FORNIX TRANSECTION; PRENATAL RAT-BRAIN; TYROSINE-HYDROXYLASE; TARGET-CELLS; CATECHOLAMINERGIC NEURONS; NOREPINEPHRINE UPTAKE;
Keywords:
NEURONAL SURVIVAL; TYROSINE HYDROXYLASE; NORADRENERGIC BRAIN NUCLEUS; MATURATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
L.J. Robinson et al., "HIPPOCAMPAL REGULATION OF THE SURVIVAL AND MORPHOLOGICAL DEVELOPMENT OF LOCUS-CERULEUS NEURONS IN DISSOCIATED CELL-CULTURE", Journal of comparative neurology, 333(4), 1993, pp. 567-577

Abstract

The influence of target structures on neural development, originally described for the peripheral nervous system, has more recently been investigated in the central nervous system. We sought to determine whether targets regulate the development of the locus coeruleus, with its diffuse and complex projections in marked contrast to the simpler neural circuits of the peripheral nervous system. Dissociated locus coeruleus cells were grown alone or with the hippocampus in serum-free, chemically defined medium that minimized non-neuronal growth. Coculture with the hippocampus resulted in a significant increase in locus coeruleus tyrosine hydroxylase activity. Elevated tyrosine hydroxylase activity was accompanied by a commensurate increase in the number of tyrosinehydroxylase-immunoreactive cells, suggesting hippocampal enhancement of locus coeruleus survival. Furthermore, when hippocampal cells were added to locus coeruleus dissociates at zero time, or after two days, tyrosine hydroxylase-positive cell number was increased only by hippocampal cells added initially, suggesting that the target does indeed foster survival. The apparent target enhancement of locus coeruleus survival seems to be selective since total protein and total neuron number, estimated with neuron-specific enolase immunocytochemistry, were notaffected by the hippocampus. Coculture with the hippocampus also increased the length and complexity of locus coeruleus cell processes. Neither the increase in tyrosine hydroxylase cell number nor the changes in morphology could be attributed to nonspecific effects of the increased cell density in cocultures. Our observations thus suggest that thetarget hippocampus influences the survival and neurite elaboration ofafferent locus coeruleus neurons.

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Documento generato il 01/04/20 alle ore 01:44:08