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Titolo:
DELETION OF DNA ENCODING THE 1ST 5 TRANSMEMBRANE DOMAINS OF EPSTEIN-BARR-VIRUS LATENT MEMBRANE PROTEIN-2A AND PROTEIN-2B
Autore:
LONGNECKER R; MILLER CL; TOMKINSON B; MIAO XQ; KIEFF E;
Indirizzi:
NORTHWESTERN UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL,303 E CHICAGO AVE CHICAGO IL 60611 HARVARD UNIV,SCH MED,DEPT MED BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET BOSTON MA 02115
Titolo Testata:
Journal of virology
fascicolo: 8, volume: 67, anno: 1993,
pagine: 5068 - 5074
SICI:
0022-538X(1993)67:8<5068:DODET1>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NASOPHARYNGEAL CARCINOMA; IMMORTALIZED LYMPHOCYTES; MARMOSET LEUKOCYTES; HODGKINS-DISEASE; GENE-PRODUCTS; CELLS; TRANSFORMATION; EXPRESSION; INFECTION; GENOME;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
R. Longnecker et al., "DELETION OF DNA ENCODING THE 1ST 5 TRANSMEMBRANE DOMAINS OF EPSTEIN-BARR-VIRUS LATENT MEMBRANE PROTEIN-2A AND PROTEIN-2B", Journal of virology, 67(8), 1993, pp. 5068-5074

Abstract

A recombinant Epstein-Barr virus (EBV) was constructed, with a positive-selection marker inserted at the site of a deletion of a DNA segment which encodes the first five transmembrane domains of LMP2A and LMP2B. Despite the mutation, the mutant recombinant EBV was able to initiate and maintain primary B-lymphocyte growth transformation in vitro. Cells transformed with the mutant recombinant were not different from wild-type virus transformants in initial or long-term outgrowth, sensitivity to limiting cell dilution, or serum requirement. Expression of EBNA1, EBNA2, EBNA3A, EBNA3C, and LMP1 and permissivity for lytic EBV infection were also unaffected by the LMP2 deletion mutation. These results complete the molecular genetic studies proving LMP2 is dispensable for primary B-lymphocyte growth transformation, latent infection, and lytic virus replication in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:00:45