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Titolo:
GENETIC-EVIDENCE THAT THE TAT PROTEINS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 AND TYPE-2 CAN MULTIMERIZE IN THE EUKARYOTIC CELL-NUCLEUS
Autore:
BOGERD HP; FRIDELL RA; BLAIR WS; CULLEN BR;
Indirizzi:
DUKE UNIV,MED CTR,HOWARD HUGHES MED INST DURHAM NC 27710 DUKE UNIV,MED CTR,HOWARD HUGHES MED INST DURHAM NC 27710 DUKE UNIV,MED CTR,GENET SECT DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT MICROBIOL DURHAM NC 27710
Titolo Testata:
Journal of virology
fascicolo: 8, volume: 67, anno: 1993,
pagine: 5030 - 5034
SICI:
0022-538X(1993)67:8<5030:GTTTPO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL DOMAINS; TRANS-ACTIVATOR; REGULATORY PROTEIN; HIV-1 TAT; EXPRESSION; BINDING; YEAST; RNA; TRANSACTIVATION; IDENTIFICATION;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
H.P. Bogerd et al., "GENETIC-EVIDENCE THAT THE TAT PROTEINS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 AND TYPE-2 CAN MULTIMERIZE IN THE EUKARYOTIC CELL-NUCLEUS", Journal of virology, 67(8), 1993, pp. 5030-5034

Abstract

The formation of dimers or higher-order multimers is critical to the biological activity of many eukaryotic regulatory proteins. However, biochemical analyses of the multimerization capacity of the Tat trans activator of human immunodeficiency virus types 1 (HIV-1) and 2 (HIV-2)have yielded contradictory results. We used the two-hybrid genetic assay for protein-protein interactions in the eukaryote Saccharomyces cerevisiae (S. Fields and O.-K. Song, Nature [London] 340:245-246, 1989)to examine the multimerization of Tat in vivo. Both HIV-1 and HIV-2 Tat are shown to form specific homo- but not heteromultimers in the yeast cell nucleus. Mutational analysis indicates a critical role for theessential core motif of Tat in mediating this interaction but demonstrates that efficient Tat multimerization does not require an intact cysteine motif. These data raise the possibility that the multimerization of Tat may be important for Tat function in higher eukaryotes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:24:21