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Titolo:
INVESTIGATION OF CARDIAC METABOLISM USING STABLE ISOTOPES AND MASS-SPECTROMETRY
Autore:
MULLER FU; HUNNEMAN DH; KAHLES R; HELLIGE G;
Indirizzi:
UNIV GOTTINGEN,ZENTRUM ANAESTHESIOL RETTUNGS & INTENSIVMED,ROBERT KOCH STR 40 W-3400 GOTTINGEN GERMANY
Titolo Testata:
Basic research in cardiology
fascicolo: 3, volume: 88, anno: 1993,
pagine: 272 - 281
SICI:
0300-8428(1993)88:3<272:IOCMUS>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
FATTY-ACID OXIDATION; 2-(3-METHYLCINNAMYLHYDRAZONO)-PROPIONATE BM 42.304; RAT-HEART; MYOCARDIAL ISCHEMIA; HYPOGLYCEMIC AGENT; BLOOD-FLOW; INFARCTION; TOMOGRAPHY; TRANSPORT; ALBUMIN;
Keywords:
STABLE ISOTOPES; MASS SPECTROMETRY; WORKING RAT HEART; METABOLISM; ACETATE; PALMITATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
F.U. Muller et al., "INVESTIGATION OF CARDIAC METABOLISM USING STABLE ISOTOPES AND MASS-SPECTROMETRY", Basic research in cardiology, 88(3), 1993, pp. 272-281

Abstract

The technique described in this communication enables detailed investigations of cardiac metabolism using C-13-labeled substrates and mass spectrometric measurements of (CO2)-C-13 in the coronary effluent. To validate this technique for further studies isolated working rat hearts were perfused with C-13-labeled substrates in a bicarbonate-free perfusion fluid. The fraction Of CO2 produced by oxidation of labeled substrate was calculated by the (CO2)-C-13/CO2 ratio in the coronary perfusate. The oxidation of C-13-acetate showed a linear correlation with C-13-acetate concentrations between 0.015 and 0.16 mmol/l. An inhibitor of acylcarnitine translocase, 2-(3-methylcinnamylhydrazono)-propionate (BM42.304) decreased CO2 production from C-13-palmitate from 48 % +/- 4 % to 31 % +/- 3 % (n = 11, SEM). Taking into account considerations of tracer kinetic theory rapidly accessible intracellular palmitatestores were estimated to be less than 900 nmol/g ww. This technique allows specific investigations of the oxidation of labeled substrates in the heart and may be useful for basic research and/or clinical diagnosis, thus avoiding the hazards of radiolabeled substrates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:15:37