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Titolo:
THE SACCHAROMYCES-CEREVISIAE PRP21 GENE-PRODUCT IS AN INTEGRAL COMPONENT OF THE PRESPLICEOSOME
Autore:
ARENAS JE; ABELSON JN;
Indirizzi:
CALTECH,DIV BIOL,147-75 PASADENA CA 91125
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 14, volume: 90, anno: 1993,
pagine: 6771 - 6775
SICI:
0027-8424(1993)90:14<6771:TSPGIA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMALL NUCLEAR RIBONUCLEOPROTEIN; PRE-MESSENGER-RNA; SPLICING INVITRO; SNRNP PROTEIN; YEAST; SPLICEOSOME; IDENTIFICATION; PARTICLE; PATHWAY; MUTATIONS;
Keywords:
SPLICING; SPLICEOSOME; U2 SMALL NUCLEAR RIBONUCLEOPROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
J.E. Arenas e J.N. Abelson, "THE SACCHAROMYCES-CEREVISIAE PRP21 GENE-PRODUCT IS AN INTEGRAL COMPONENT OF THE PRESPLICEOSOME", Proceedings of the National Academy of Sciences of the United Statesof America, 90(14), 1993, pp. 6771-6775

Abstract

In Saccharomyces cerevisiae, the prp21 mutation causes accumulation of unspliced pre-mRNA at the nonpermissive temperature. We have cloned the PRP21 gene by complementation of its temperature-sensitive phenotype and found it to be the same as SPP91, an extragenic suppressor of the prp9 mutation previously studied in vivo by Chapon and Legrain [Chapon, C. & Legrain, P. (1992) EMBO J. 11, 3279-3288]. We have analyzed the effects of the prp21 mutation on splicing in vitro and have found that PRP21 is a splicing factor required for prespliceosome assembly. We also have analyzed the interaction of PRP21 with splicing complexesusing anti-PRP21 antibodies and found that the RNA components of the prespliceosome-U1 and U2 small nuclear RNA (snRNA) particles and pre-mRNA-are specifically coimmunoprecipitated under splicing conditions inthe presence of 0.2 M KCl. At higher KCl concentrations, U1 snRNP dissociates from splicing complexes; nevertheless, U2 snRNA and pre-mRNA are still efficiently immunoprecipitated. Immunoprecipitation of both U1 and U2 snRNA as well as pre-mRNA is ATP-dependent and requires a pre-mRNA capable of supporting prespliceosome assembly. Analysis of the unbound complexes in native gels confirmed that prespliceosomes are specifically immunoprecipitated by anti-PRP21 antibodies. These results demonstrate that PRP21 is an integral component of the prespliceosome and establishes a stable interaction with U2 snRNP and/or pre-mRNA in that complex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 00:51:50