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Titolo:
SEQUENCE-ANALYSIS OF THE BETA-N-ACETYLHEXOSAMINIDASE GENE OF VIBRIO-VULNIFICUS - EVIDENCE FOR A COMMON EVOLUTIONARY ORIGIN OF HEXOSAMINIDASES
Autore:
SOMERVILLE CC; COLWELL RR;
Indirizzi:
UNIV MARYLAND,MARYLAND BIOTECHNOL INST,CTR MARINE BIOTECHNOL COLL PK MD 20742 UNIV MARYLAND,MARYLAND BIOTECHNOL INST,CTR MARINE BIOTECHNOL COLL PK MD 20742
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 14, volume: 90, anno: 1993,
pagine: 6751 - 6755
SICI:
0027-8424(1993)90:14<6751:SOTBGO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID SEQUENCE; SIMILARITY SEARCHES; EXTENSIVE HOMOLOGY; ALPHA-CHAIN; CLONING; CDNA; SUBUNIT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
C.C. Somerville e R.R. Colwell, "SEQUENCE-ANALYSIS OF THE BETA-N-ACETYLHEXOSAMINIDASE GENE OF VIBRIO-VULNIFICUS - EVIDENCE FOR A COMMON EVOLUTIONARY ORIGIN OF HEXOSAMINIDASES", Proceedings of the National Academy of Sciences of the United Statesof America, 90(14), 1993, pp. 6751-6755

Abstract

DNA cloned from the marine bacterium Vibrio vulnificus into Escherichia coli HB101 can hydrolyze chitin oligomer analogs in the recipient. The nucleotide sequence of the cloned DNA was determined and a single long open reading frame of 2541 base pairs (initiation codon through termination codon) was found. The nucleotide sequence predicts a gene product of 847 amino acids and a molecular mass of 94.3 kDa. In vitro transcription and translation analyses indicated a single protein of 94kDa encoded by the cloned DNA. The gene product hydrolyzes methylumbelliferyl beta-D Conjugates of chitotriose, chitobiose, N-acetylglucosamine, and N-acetylgalactosamine and has, therefore, been termed a beta-N-acetylhexosaminidase. The predicted protein shares a high degree ofsequence similarity with the chitobiase of Vibrio harveyi and limitedsimilarity with the alpha chain of human beta-hexosaminidase. Clusteranalyses suggest a common evolutionary ancestor for all known hexosaminidase enzymes, with no detectable relationship to known chitinases.

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Documento generato il 01/12/20 alle ore 13:34:36